Stein

Effects of adding HCl to collection bags or collection pitchers on amino acid digestibility in corn, soybean meal, or wheat middlings fed to growing pigs

In most AA digestibility experiments collection bags are changed at least every 30 min and the collected ileal digesta are stored at –20 °C to prevent microbial degradation of proteins. Other attempts to reduce microbial activity in digesta include adding acids to collection bags to reduce the pH in ileal digesta. Acids may also be added to collection pitchers. However, in some experiments, no attempt to reduce pH is made because it is assumed that the rapid reduction in temperature after collection is sufficient to prevent microbial degradation of proteins.

To our knowledge, there is, however, no information about the necessity of adding acids to collected digesta and it is not known if values for apparent ileal digestibility (AID), basal endogenous losses of AA, or standardized ileal digestibility (SID) of AA are influenced by addition of acids to digesta. Therefore, the objective of this experiment was to test the null-hypothesis that values for AID, basal endogenous losses, and SID of AA are not influenced by addition of acids to ileal digesta collection bags or to collection pitchers.

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Protein quality in ready-to-use supplementary foods for moderate wasting

Roediger, Rebecca, Hans-Henrik Stein, Meghan Callaghan-Gillespie, Jeffrey Kahn Blackman, Kristin Kohlmann, Kenneth Maleta, Mark Manary. 2020. Protein quality in ready-to-use supplementary foods for moderate wasting. Matern Child Nutr. 2020;e13019. doi.org/10.1111/mcn.13019. Link to the full text.

The direct and difference procedures result in similar estimates for amino acid digestibility in feed ingredients fed to growing pigs

Oliveira, Maryane S. F., John K. Htoo, and Hans H. Stein. 2020. The direct and difference procedures result in similar estimates for amino acid digestibility in feed ingredients fed to growing pigs. Journal of Animal Science, 2020, Vol. 98, No. 8, 1–8. doi:10.1093/jas/skaa225. Link to full text.

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Values for digestible indispensable amino acid score (DIAAS) determined in pigs for breakfast cereals and milk are additive in combined breakfast cereal-milk meals

Breakfast cereals are usually consumed with milk as a breakfast meal. To meet requirements for amino acids (AA), higher quality proteins are needed to complement the protein in cereals to provide a meal that is adequate in all indispensable AA. The digestible indispensable amino acid score (DIAAS) method used to determine protein quality allows for calculation of the protein value of individual ingredients and mixed meals consisting of several proteins. Values for DIAAS are based on values for apparent ileal digestibility (AID) of AA that are corrected for the basal endogenous loss of each individual AA, resulting in values defined as standardized ileal digestibility (SID). Values for SID of AA are additive in mixed meals because these values are independent of basal endogenous losses. Consequently, it is expected that DIAAS obtained for individual food ingredients are additive in a mixed meal, but data to demonstrate this have not been reported. Therefore, the objective of this experiment was to test the hypothesis that AA in milk complement AA in breakfast cereals to provide a balanced meal and that DIAAS in individual foods are additive in a combined meal.  

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Determination of true ileal amino acid digestibility in the growing pig for calculation of digestible indispensable amino acid score (DIAAS)

Hodgkinson, Suzanne M., Hans H. Stein, Sonja de Vries, Wouter H. Hendriks, and Paul J. Moughan. 2020. Determination of true ileal amino acid digestibility in the growing pig for calculation of digestible indispensable amino acid score (DIAAS). The Journal of Nutrition. First published online, 2020. doi.org/10.1093/jn/nxaa210.

Digestibility of amino acids and concentrations of metabolizable energy and net energy are greater in high-shear dry soybean expellers than in soybean meal when fed to growing pigs

Rodriguez, Diego A., Su A Lee, and Hans H. Stein. 2020. Digestibility of amino acids and concentrations of metabolizable energy and net energy are greater in high-shear dry soybean expellers than in soybean meal when fed to growing pigs. Journal of Animal Science, 2020, Vol. 98, No. 7, 1–8.

doi:10.1093/jas/skaa215. Link to full text.

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Excessive heating of 00-rapeseed meal reduces not only amino acid digestibility but also metabolizable energy when fed to growing pigs

Oliveira, Maryane S. F., Markus K. Wiltafsky-Martin, and Hans H. Stein. 2020. Excessive heating of 00-rapeseed meal reduces not only amino acid digestibility but also metabolizable energy when fed to growing pigs. Journal of Animal Science, 2020, Vol. 98, No. 7, 1–9. doi:10.1093/jas/skaa219. Link to full text.

The apparent ileal digestibility and the apparent total tract digestibility of carbohydrates and energy in hybrid rye are different from some other cereal grains when fed to growing pigs

McGhee, Molly L., and Hans. H. Stein. 2020. The apparent ileal digestibility and the apparent total tract digestibility of carbohydrates and energy in hybrid rye are different from some other cereal grains when fed to growing pigs. Journal of Animal Science, 2020, Vol. 98, No. 7, 1–10. doi:10.1093/jas/skaa218. Link to full text.

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Reduce crude protein to improve fecal consistency, intestine morphology

Limbach, Joseph R., Charmaine D. Espinosa, and Hans H. Stein. 2020. Reduce crude protein to improve fecal consistency, intestine morphology. National Hog Farmer, Online edition, July 30, 2020. Link to full text.

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Effects of concentration of calcium and phosphorus and 1-alpha-hydroxycholecalciferol (1-α-OH-D3) on digestibility and retention of calcium and phosphorus and concentration of digestible energy in diets fed to sows in late-gestation

Absorption of Ca and P by active transport in the small intestine is regulated by calcitriol, which is the active form of vitamin D (1,25-dihydroxycholecalciferol) and the hormones calcitonin and PTH. One-alpha-hydroxycholecalciferol (1-α-OH-D3) is an active vitamin D3 analog that does not require the second hydroxylation step for vitamin D3 to be active. It is possible that supplementation of 1-α-OH-D3 may increase absorption of Ca and P.

The requirement for Ca and P by gestating sows increases in late gestation compared with early- and mid-gestation because of increased needs by the developing fetuses. It is also possible that dietary concentrations of Ca and P affect the rate of absorption of Ca and P in sows but data to demonstrate this have not been reported. Therefore, the objective of this experiment was to test the hypothesis that the Ca and P concentrations in diets fed to gestating sows in late gestation and supplementation of 1-α-OH-D3 affect apparent total tract digestibility (ATTD) and retention of Ca and P as well as the ATTD of GE and concentration of DE in diets. The second hypothesis was that there is an interaction between dietary Ca and P concentrations and supplementation of 1-α-OH-D3 in diets fed to gestating sows.

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A new source of high-protein distillers dried grains with solubles (DDGS) has greater digestibility of amino acids and energy, but less digestibility of phosphorus, than de-oiled DDGS when fed to growing pigs

Cristobal, Minoy, Jessica P. Acosta, Su A Lee, and Hans H. Stein. 2020. A new source of high-protein distillers dried grains with solubles (DDGS) has greater digestibility of amino acids and energy, but less digestibility of phosphorus, than de-oiled DDGS when fed to growing pigs. Journal of Animal Science, 2020, Vol. 98, No. 7, 1–9. doi:10.1093/jas/skaa200.

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Digestibility of amino acids, fiber, and energy by growing pigs, and concentrations of digestible and metabolizable energy in yellow dent corn, hard red winter wheat, and sorghum may be influenced by extrusion

Rodriguez, Diego A., Su A. Lee, Cassandra K. Jones, John K. Htoo, Hans H. Stein. 2020. Digestibility of amino acids, fiber, and energy by growing pigs, and concentrations of digestible and metabolizable energy in yellow dent corn, hard red winter wheat, and sorghum may be influenced by extrusion. Animal Feed Science and Technology 268 (2020) 114602. https://doi.org/10.1016/j.anifeedsci.2020.114602.

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Effects of particle size reduction, pelleting, and extrusion on the nutritional value of ingredients and diets fed to pigs: A review

Lancheros, J. P., C. D. Espinosa, H. H. Stein. 2020. Effects of particle size reduction, pelleting, and extrusion on the nutritional value of ingredients and diets fed to pigs: A review. Animal Feed Science and Technology 268 (2020) 114603. https://doi.org/10.1016/j.anifeedsci.2020.114603.

Effect of formulating diets based on a ratio between STTD Ca and STTD P and the inclusion of phytase on the calcium and phosphorus balance of growing pigs

Several experiments were conducted to estimate Ca digestibility in different feed ingredients in the presence or absence of microbial phytase to allow formulation of diets for pigs to be based on standardized total tract digestible (STTD) Ca instead of total Ca. Thus, 4 experiments aimed at determining Ca requirements expressed as a ratio between STTD Ca and STTD P in pigs from 11 to 130 kg. A follow-up study was later conducted to validate those data and to evaluate the effect of using ratios that maximize growth performance on bone development because maximum bone ash requires more Ca than maximum growth performance. However, data indicate that STTD Ca to STTD P ratios to maximize Ca retention are greater than to maximize bone ash synthesis. The use of STTD Ca to STTD P ratios in diet formulation may result in a reduction in excess dietary Ca, which is beneficial because excess dietary Ca is detrimental to P digestibility and growth performance of pigs. Therefore, the objective of this experiment was to test the hypothesis that formulating diets for growing pigs based on a ratio between STTD Ca and STTD P instead of total Ca and STTD P does not decrease Ca retention, but increases P utilization.

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Pigs prefer diets containing corn to diets containing hybrid rye when given the choice, but growth performance is not reduced when hybrid rye replaces corn in diets for growing pigs

In many parts of the world, including the United States, corn is the primary energy source used in diets for pigs, but there are no published data comparing the growth performance of growing pigs fed diets in which hybrid rye replaces corn. Unfamiliarity with hybrid rye also makes some producers in the United States reluctant to try feeding hybrid rye to pigs, as there is a long-standing belief that rye is less palatable than other feed ingredients. Therefore, two experiments were conducted to test the hypotheses that there is no difference in feed preference for diets containing either hybrid rye or corn as the exclusive cereal grain source, and that hybrid rye may replace a portion of corn in diets for growing pigs without adversely affecting growth. 

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Raw and roasted pistachio nuts (Pistacia vera L.) are ‘good’ sources of protein based on their digestible indispensable amino acid score as determined in pigs

Bailey, Hannah M., and Hans H. Stein. 2020. Raw and roasted pistachio nuts (Pistacia vera L.) are ‘good’ sources of protein based on their digestible indispensable amino acid score as determined in pigs. J Sci Food Agric 2020; 100: 3878–3885. Link to full text.

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Effects of isoquinoline alkaloids on apparent ileal digestibility of amino acids, acid hydrolyzed ether extract, and starch by young growing pigs fed corn-soybean meal diets

Rundle, Carly M., Valeria Artuso-Ponte, and Hans H. Stein. 2020. Effects of isoquinoline alkaloids on apparent ileal digestibility of amino acids, acid hydrolyzed ether extract, and starch by young growing pigs fed corn-soybean meal diets. Transl. Anim. Sci. 2020.4:1-7. doi: 10.1093/tas/txaa054. Link to full text. 

Most meat products have digestible indispensable amino acid scores that are greater than 100, but processing may increase or reduce protein quality

Bailey, Hannah M., John K. Mathai, Eric P. Berg, and Hans H. Stein. 2020. Most meat products have digestible indispensable amino acid scores that are greater than 100, but processing may increase or reduce protein quality. British Journal of Nutrition (2020), 124, 14–22. doi:10.1017/S0007114520000641. Link to full text.

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Impact of increasing the levels of insoluble fiber and on the method of diet formulation measures of energy and nutrient digestibility in growing pigs

Acosta, Jesus A., Hans H. Stein, and John F. Patience. 2020. Impact of increasing the levels of insoluble fiber and on the method of diet formulation measures of energy and nutrient digestibility in growing pigs. Journal of Animal Science, 2020, 1–9. doi:10.1093/jas/skaa130. Link to full text.

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Nutritional evaluation of different varieties of sorghum and the effects on nursery pig growth performance

Thomas, Lori L., Charmaine D. Espinosa, Robert D. Goodband, Hans H. Stein, Mike D. Tokach, Steve S. Dritz, Jason C. Woodworth, and Joel M. DeRouchey. 2020. Nutritional evaluation of different varieties of sorghum and the effects on nursery pig growth performance. Journal of Animal Science, 2020, 1–16.  doi:10.1093/jas/skaa120.

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