Research Reports

Effects of super dosing 4 different sources of phytase on amino acid digestibility

Dietary phytate may bind to proteins from feed ingredients by making indigestible nutrient-complexes. Therefore, it is possible that adding exogenous phytase to the diets increases digestibility of amino acids (AA). However, results of experiments in which microbial phytase has been added to diets fed to pigs have not consistently demonstrated increased ileal digestibility of AA. It is, however, possible that is because the dose of phytase was too low to obtain a positive effect on AA digestibility and that if greater doses were used, a positive response would be obtained. Therefore, the objective of this experiment was to test the hypothesis that super dosing four different sources of commercially available exogenous phytase increases the apparent ileal digestibility (AID) of CP and AA in a corn-soybean meal (SBM) based diet fed to growing pigs.

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Effects of intrinsic phytase from hybrid rye on P and Ca digestibility in a corn-soybean meal diet

Rye contains considerable quantities of intrinsic phytase and the presence of phytase in rye may result in an increased P digestibility in pigs without using exogenous phytase. It is possible that the endogenous phytase in rye also increases P digestibility in other plant feed ingredients by releasing P from the phytate. However, to our knowledge, no data demonstrating this effect have been published. Therefore, the objective of this experiment was to test the hypothesis that inclusion of rye in diets containing corn and soybean meal (SBM) without or with microbial phytase improves the apparent total tract digestibility (ATTD) of P and Ca and thus the ATTD of P is not additive in the mixed diet.

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Effects of inclusion of hybrid rye in diets on growth performance and diarrhea incidence of weanling pigs

Production of hybrid rye in North America is increasing after being introduced to Canada and the United States in 2014 and 2016, respectively. Compared with corn, hybrid rye contains similar amounts of standardized ileal digestible amino acids, a greater concentration of standardized total tract digestible P, and approximately 94% of the metabolizable energy. Hybrid rye contains more fermentable dietary fiber than corn, which has the potential to improve gut health, but its reduced digestibility of amino acids may simultaneously have a negative impact on the health of the large intestine. Two experiments were conducted to determine the maximum amount of hybrid rye that can be included in diets for weanling pigs without influencing growth performance or diarrhea incidence.

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Digestibility of energy, and total dietary fiber, and concentration of digestible and metabolizable energy in soybean expeller and soybean meal fed to growing pigs

When soybeans are crushed, the oil may be solvent extracted and the resulting defatted soybeans are known as soybean meal (SBM). However, the oil may also be mechanically expelled from the beans and the defatted soybeans resulting from this procedure is an ingredient known as soybean expellers. The expelling procedure is less efficient in removing oil from the beans and soybean expellers, therefore, contain more residual oil than SBM. Soybean expellers can be produced using different technologies, and a new procedure involving a patented high shear dry extrusion procedure was recently developed (Insta-Pro International, Des Moines, IA). There are however, no data for the apparent total tract digestibility (ATTD) of GE and total dietary fiber (TDF) and DE and ME concentrations in the soybean expellers that are produced from this procedure. Therefore, the objective of this research was to test the hypothesis that the ATTD of GE and TDF and concentrations of DE and ME are greater in soybean expellers than in SBM when fed to growing pigs.

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Effects of dietary leucine and tryptophan supplementation on serotonin metabolism and growth performance of growing pigs

Tryptophan is an indispensable AA that is often limiting for growth in pigs fed corn-soybean meal-based diets. Tryptophan may act as a regulator of feed intake by enhancing serotonin signaling in the brain, because Trp is a precursor for serotonin. High Trp intake increases feed intake, and this is partly attributed to increased serotonin synthesis. Availability of dietary Trp in the brain is considered the rate-limiting step in hypothalamic serotonin synthesis. However, to be transported into the brain, Trp competes with other large neutral AA such as Val, Leu, Ile, Tyr, and Phe for a common transporter (L-type AA transporter 1) to cross the blood-brain barrier.

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Digestibility of amino acids is greater in soybean expellers than in soybean meal when fed to growing pigs

When soybeans are crushed, the oil may be solvent extracted and the resulting defatted soybeans are known as soybean meal (SBM). However, the oil may also be mechanically expelled from the beans and the defatted soybeans resulting from this procedure is an ingredient known as soybean expellers. The expelling procedure is less efficient in removing oil from the beans and soybean expellers, therefore, contain more residual oil than SBM. Soybean expellers can be produced using different technologies, and a new procedure involving a patented high shear dry extrusion procedure was recently developed (Insta-Pro International, Des Moines, IA). There are however, no data for the digestibility of amino acids (AA) in the soybean expellers that are produced from this procedure. Therefore, the objective of this research was to test the hypothesis that the standardized ileal digestibility (SID) of crude protein (CP) and AAis greater in soybean expellers than in SBM when fed to growing pigs.

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Digestibility of energy and phosphorus and concentration of metabolizable energy in a new source of high-protein distillers dried grains fed to growing pigs

Recently a high-protein distillers dried grains (HP-DDGS; ProCap DDGS, Marquis Energy, Hennepin, IL) was developed and the HP-DDGS has greater concentrations of CP and fat, but contains less fiber compared with conventional DDGS, which may affect the digestibility of energy and P and concentrations of DE and ME. There are, however, no data for the nutritional value of this new source of DDGS. Therefore, 2 experiments were conducted to test the hypothesis that concentrations of DE and ME, and standardized total tract digestibility (STTD) of P in HP-DDGS are greater than in conventional DDGS.

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Effects of copper hydroxychloride and dietary fiber on intestinal permeability, growth performance, and blood characteristics of nursery pigs

Most diets for weanling pigs contain highly digestible plant and animal proteins, but there is an increasing trend to include more fibrous co-products in diets for pigs due to reduced diet costs. However, feeding diets to nursery pigs with high concentration of dietary fiber may reduce nutrient digestibility, induce intestinal inflammation, and subsequently depress growth performance. Addition of 100 to 200 mg/kg of Cu from Cu hydroxychloride (IntelliBond CII; Micronutrients USA LLC; Indianapolis, IN) to diets improves feed efficiency and reduces post weaning diarrhea in pigs. However, there are at this point no data to demonstrate the effect of Cu hydroxychloride on intestinal barrier integrity of pigs fed low-fiber or high-fiber diets, and it is not known if Cu hydroxychloride influences immune responses of pigs. Therefore, 2 experiments were conducted to test the hypothesis that inclusion of 150 mg/kg of Cu from Cu hydroxychloride reduces intestinal permeability and subsequently improves growth performance of pigs fed diets without or with high concentration of dietary fiber.

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Digestibility of amino acids in a new source of distillers dried grains with solubles fed to growing pigs

Distillers dried grains with solubles (DDGS) is a co-product from the dry-grind ethanol production. However, quality and nutrient content of DDGS may differ among sources and origins and new technologies allow for production of DDGS with different chemical compositions as well as nutritional values. Recently a high-protein distillers dried grains (HP-DDG; ProCap DDGS, Marquis Energy, Hennepin, IL) was introduced and the HP-DDG has greater concentrations of CP and fat, but contains less fiber compared with conventional DDGS, which may affect the digestibility of AA in HP-DDG compared with conventional DDGS. There are, however, no data for the digestibility of AA in this new source of DDGS. Therefore, the objective of this research was to test the hypothesis that standardized ileal digestibility (SID) of AA in HP-DDG is greater than in conventional DDGS when fed to growing pigs.

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Effects of copper hydroxychloride on growth performance and abundance of genes involved in lipid metabolism of growing pigs

Supplementing Cu to diets fed to pigs at 100 to 250 mg/kg may reduce post-weaning scouring and also improve ADG and ADFI. Addition of Cu at 250 mg/kg in diets for pigs containing 5% animal fat improved growth performance, and it was speculated that this is due to the ability of Cu to improve animal fat utilization and enzymatic activity. Inclusion of 45 mg/kg of dietary Cu in diets for rabbits improved body mass gain by upregulating mRNA transcription of fatty acid transport protein, fatty acid binding protein, and carnitine palmitoyl transferase 1, indicating that dietary Cu may influence post-absorptive metabolism of lipids. However, the effect of supplementing dietary Cu on post-absorptive lipid metabolism in pigs remains inconclusive. Therefore, an experiment was conducted to test the hypothesis that addition of 150 mg/kg Cu from Cu hydroxychloride (IntelliBond CII; Micronutrients USA LLC; Indianapolis, IN) to a diet based on corn, soybean meal (SBM), and distillers dried grains with solubles (DDGS) improves growth performance of pigs, and that dietary Cu influences mRNA abundance of genes involved in post-absorptive metabolism of lipids in pigs.

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Effect of inclusion of an enhanced torula yeast on growth performance, fecal score, and blood characteristics of weanling pigs

Digestibility experiments have been conducted to determine the nutritional value of an enhanced torula yeast derived from forestry by-products. Results of these experiments indicated that enhanced torula yeast has a greater digestibility of amino acids and P than fish meal, and a concentration of metabolizable energy that is not different from that in fish meal. Therefore, it is believed that the enhanced torula yeast can be included in diets for weanling pigs at the expense of fish meal and other animal protein sources. Therefore, the objective of this experiment was to determine the effects on growth performance, fecal score, and blood characteristics of including the enhanced torula yeast at the expense of fish meal and plasma protein in diets fed to weanling pigs.

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Phosphorus and energy digestibility of Fermex 200 (fermented soybean meal) fed to weanling pigs

Soybean meal (SBM) is one of the most important protein sources in swine diets. However, most P in SBM is bound to phytate, which increases inclusion of inorganic P in diets for pigs. Use of microbial phytase may hydrolyze phytate and subsequently improve P absorption. Fermex 200 (Purina Animal Nutrition, Shoreview, MN, USA) is a new source of fermented SBM that may serve as an alternative to other protein sources in diets fed to pigs. However, there are at this point no data for effects of adding phytase to diets containing Fermex 200 and no data for digestible energy (DE) and metabolizable energy (ME) concentrations of Fermex 200.

Therefore, 2 experiments were conducted to test the hypothesis that inclusion of 1,000 phytase units (FTU)/kg of microbial phytase improves the standardized total tract digestibility (STTD) of P in conventional SBM and Fermex 200. The second hypothesis was that the STTD of P, as well as concentrations of DE and ME in Fermex 200 are greater than in conventional SBM.

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Evaluation of adaptation times and indigestible markers in ileal digestibility studies for pigs

Values for digestibility of nutrients in diets or feed ingredients fed to pigs are determined by nutrient intake and nutrient excretion in feces or ileal digesta. Ileal nutrient digestibility is determined using an indigestible marker because complete quantitative collection of ileal digesta is usually not possible. As a consequence, analysis of marker concentration in digesta is critical for accurate calculation of digestibility coefficients. Therefore, a sufficient adaptation period before initiation of ileal digesta collection is also important to make sure that the concentration of the marker in digesta is constant. It is, however, not known if the adaptation period is different among different markers. Therefore, an experiment was conducted to test the hypothesis that adaptation is needed to have constant marker concentrations in ileal digesta from pigs. The second objective was to test the null-hypothesis that apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of AA digestibility and the basal endogenous losses of AA are constant regardless of the indigestible marker used.

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Effects of Fermex 200 (fermented soybean meal) on growth performance and amino acid digestibility by weanling pigs

Soybean meal (SBM) is one of the most important protein sources in swine diets. However, SBM has high concentrations of non-digestible oligosaccharides, mainly stachyose, raffinose, and verbascose, which may increase diarrhea incidence and reduce nursery growth performance. Fermex 200 (Purina Animal Nutrition, Shoreview, MN, USA) is a new source of fermented SBM that may serve as an alternative to other protein sources in diets fed to pigs. However, there are at this point no data for effects of Fermex 200 on growth performance and amino acid (AA) digestibility when fed to weanling pigs.

Therefore, 2 experiments were conducted to test the hypothesis that the apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of AA in Fermex 200 are greater than in conventional SBM. The second hypothesis was that Fermex 200 supports growth of weanling pigs as well as other protein sources.

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Digestibility of phosphorus in enhanced torula yeast and fish meal fed to weanling pigs

An enhanced torula yeast was recently developed as a natural alternative protein source to fish meal and other animal proteins. This ingredient has a lower carbon footprint than other available yeast proteins because it is derived from forestry by-products. The enhanced torula yeast has an improved amino acid (AA) profile, a greater digestibility of AA than fish meal, and an energy value that is not different from that in fish meal. However, there are no data available about the digestibility values of P in this new ingredient. Therefore, an experiment was conducted to test the hypothesis that the values for apparent total tract digestibility (ATTD) and standardized total tract digestibility (STTD) of P in enhanced torula yeast are not different from those in fish meal.

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Amino acid digestibility in plant based protein concentrates and in Peruvian super prime fish meal fed to weanling pigs

Anti-nutritional factors in soybean meal can result in a reduction in digestibility of amino acids. Therefore, the use of soybean meal is limited in diets for weanling pigs and fish meal and other animal protein sources are often used instead. However, technologically treated plant proteins or mixtures of proteins may also be used instead of animal proteins if the treatments employed have reduced or eliminated the anti-nutritional factors. An example of 2 new plant-based proteins are protein concentrate 56 (JPC 56) and Fish Meal Replacer Omega 3 (FMR Ω 3). Both proteins are produced by Joosten, (Ab Weert, The Netherlands), but there is no information about the digestibility of amino acids in these proteins. Therefore, an experiment was conducted to test the hypothesis that the apparent ileal digestibility (AID) and the standardized ileal digestibility (SID) of amino acids in JPC 56 and FMR Ω 3 are not different from AID and SID values in fish meal.

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Effects of copper hydroxychloride and distillers dried grains with solubles on intestinal microbial protein concentration and digestibility of energy, crude protein, and acid hydrolyzed ether extract by growing pigs

The requirement for Cu for normal metabolism by weanling pigs is 5 to 6 mg/kg, but it is common practice to include additional Cu in diets for pigs to enhance growth performance. Several modes of action for the improved growth performance have been proposed, and one proposed mode of action is the ability of Cu to alter microbial activity. The apparent total tract digestibility (ATTD) of acid hydrolyzed ether extract (AEE) is improved if Cu hydroxychloride is supplemented to high fiber diets. Therefore, an experiment was conducted to test the hypothesis that inclusion of 150 mg/kg of Cu from Cu hydroxychloride (IntelliBond CII; Micronutrients USA LLC; Indianapolis, IN) to diets fed to growing pigs improves apparent ileal digestibility (AID) and ATTD of AEE, and the AID of crude protein. The second objective was to test the hypothesis that supplementing diets with Cu hydroxychloride can reduce the concentration of microbial protein in the small intestine or in the large intestine by pigs fed a corn-soybean meal diet or a diet based on corn, soybean meal, and distillers dried grains with solubles (DDGS).

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Amino acid and energy digestibility of an enhanced torula yeast and fish meal fed to weanling pigs

Yeast may be used instead of fish meal or other animal protein sources in diets for weanling pigs and there are several yeast proteins available. A newly developed enhanced torula yeast is derived from forestry by-products, and therefore, has a lower carbon-footprint compared with other yeast products. This enhanced torula yeast has an improved amino acid (AA) profile compared with traditional fermentation products, but at this time no data for the nutritional value of enhanced torula yeast fed to weanling pigs have been published. Therefore, 2 experiments were conducted to test the hypothesis that the AA and energy values of enhanced torula yeast is not different from that of fish meal. In Exp. 1, the apparent ileal digestibility (AID) and the standardized ileal digestibility (SID) of AA were determined, whereas Exp. 2 was designed to determine the concentration of digestible energy (DE) and metabolizable energy (ME) in both enhanced torula yeast and fish meal.

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Excessive heat treatment of distillers dried grains with solubles reduces not only amino acid digestibility but also concentrations of metabolizable energy when fed to growing pigs

Distillers dried grains with solubles (DDGS) is a co-product of ethanol production that is often used in diets fed to pigs because of a relatively high concentration of amino acids (AA), energy, and digestible phosphorus. However, during the cooking and drying steps, the high temperature and concentration of moisture makes DDGS susceptible to the Maillard reactions, resulting in formation of sugar-amino acid complexes. Maillard reaction products result in reduced standardized ileal digestibility (SID) of amino acids (AA), with Lys being the most sensitive AA. However, there is limited information about how heating affects the concentration of digestible energy (DE) and metabolizable energy (ME). Therefore, the objective of these experiments were to test the hypothesis that both the degree of heating and the time that heat is applied will affect the concentration of DE and ME and the SID of AA in DDGS fed to growing pigs.

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Effect of decreasing protein levels in diets fed to weanling pigs on growth performance, fecal score, and carcass characteristics

Diarrhea is one of the main problems for pigs during the post-weaning period. Traditionally, antibiotic growth promoters have been used to control post-weaning diarrhea, but consumers are increasingly concerned about this practice and there is therefore an interest in feeding diets that contain no antibiotics. However, feeding pigs without antibiotic growth promoters requires alternative strategies to control post-weaning diarrhea, but feeding low protein diets may be one way to reduce the incidence of post-weaning diarrhea. However, there is a lack of knowledge about consequences of reducing the protein level in diets fed to weanling pigs.

Feeding low protein diets to pigs results in increased net energy in the diet, reduced water intake by pigs, and reduced nitrogen excretion. This will result in reduced volume of manure and also reduced concentrations of ammonium in manure. However, if formulation of low protein diets results in feeding diets with concentrations of indispensable AA that are below the requirements, deposition of protein in pigs may be greater and deposition of fat may be increased compared with pigs fed a diet containing higher level of protein. However, it is not known if feeding a diet low in protein to weanling pigs also results in changes in carcass characteristics of market pigs.

Therefore, the objective of this experiment was to test the hypothesis that feeding a low-protein diet to pigs during the post-weaning period will result in reduced diarrhea during this period, but no effects on growth performance from wean to finish and no changes in carcass composition of pigs when they reach market weight.

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