I'm Dr. Rommel Sulabo from the Hans Stein Monogastric Nutriton Laboratory here at the University of Illinois, and today I will be discussing one of our recent research studies evaluating the amino acid digestibility of blood meal fed to weanling pigs. Dried blood meal is an animal protein product used more commonly in nursery pig diets. Numerous growth studies have demonstrated that blood meal can be a better protein source than other commonly used specialty protein sources in starter diets, such as dried skim milk, select menhaden fish meal, and soy protein concentrate. However, these growth effects of blood meal vary across studies, which may be attributed to large differences in protein quality among blood meals. To produce blood meal, whole or coagulated blood is dried in a variety of processing methods and may be sourced from either porcine, bovine, or avian origin. Thus, the nutritional value of blood meal may depend on the drying procedure or animal origin. Currently, there is limited data on the digestibility of crude protein and amino acids in blood meals produced using different drying methods and animal origin. The current NRC only reported true ileal digestibility values of amino acids for spray- or ring-dried blood meal. Therefore, the objective of this project was to measure the apparent and standardized ileal digestibility of amino acids in two sources of blood meal fed to weanling pigs. For this study, we used a total of eight weanling barrows that were equipped with a T-cannula in the distal ileum and allotted to a replicated 4x4 Latin square design with four diets and four periods in each square. There were four diets in this study. The first diet was a casein-based diet, and two diets were based on a mixture of casein and each source of blood meal. We compared two blood meal sources. The first was a spray-dried blood meal of porcine origin produced by Belstra Milling out of Demotte, Indiana, and the second was drum-dried blood meal of bovine origin produced by Cargill out of Skylar, Nebraska. The Cargill product was processed using a continuous drum dryer set at about 80 degrees Celsius with a retention time of about 20 minutes. The last diet was a nitrogen-free diet that was used to measured basal endogenous losses of amino acids and protein. All of the diets contained 0.4% chromic oxide to serve as our indigestible marker. All of the pigs in this study were fed at the level of 2.5 times the maintenance energy requirement, and water was made available at all times throughout the study. The experimental diets were fed for a period of seven days, with the initial five days serving as our adaptaton period to the diet, while days six to seven was devoted for ileal digesta collection. At the end of the study, all diets and ileal digesta samples were prepared and analyzed for dry matter, chromium, crude protein, and amino acids to calculate for both apparent and standardized ileal digestibility of crude protein and amino acids in the two blood meal sources. Now, let's discuss the results of this study. We observed that both blood meals contained high levels of crude protein, lysine, and valine, regardless of the drying method used or animal origin, which conformed with other previous reports. The spray-dried porcine blood meal contained about 98% crude protein, while the drum-dried bovine blood meal contained about 95% crude protein. The two blood meal sources also contained similar levels of indispensable and dispensable amino acids. The total lysine concentration of the two blood meal sources was 7.7 to 8.6%, which is within the range observed in other studies. However, the drum-dried bovine blood meal contained twice the concentration of methionine in spray-dried porcine blood meal. As expected, isoleucine had the lowest concentration among all indispensable amino acids for both blood meal sources, ranging between 0.42 to 0.55%. However, these were lower than those previously reported. After isoleucine, cysteine and methionine were the next limiting amino acids in both blood meal sources. Except for isoleucine, the digestibility of all indispensable amino acids in the two blood meal sources were relatively high, ranging between 90-96%, and 80-92% in spray-dried porcine and drum-dried bovine blood meal respectively. The standardized ileal digestibility of isoleucine was 63 and 72% for the spray-dried and drum-dried blood meal respectively. More importantly, the standardized ileal digestibility value of lysine was 90 to 96% for the two blood meal sources. This conformed with the values reported by NRC, which is 94% true ileal digestible lysine in spray- or ring-dried blood meal. However, the SID of lysine was lower for the drum-dried bovine blood meal than the spray-dried porcine blood meal. This suggest that the drying method used to produce the two blood meals may have affected the digestibility of heat-labile amino acids, especially lysine. Though SID values of most indispensable amino acids were greater for the spray-dried porcine blood meal, the digestible amino acid contents were similar between the two blood meal sources. This is mainly due to the higher total concentration of most indispensable amino acids in drum-dried bovine blood meal. There may be differences in amino acid concentrations between animal origins of blood meal. However, there is a wide variation in amino acid content of blood meal within each origin. For example, the lysine and the isoleucine content of bovine blood meal ranged from 7.66 to 8.59% and 0.42 to 1.04% respectively. Whereas porcine blood meal contained 7.53 to 7.73% lysine and 0.55 to 0.91% isoleucine. Previously, research from Kansas State University compared spray-dried blood meals from different animal origins and observed no differences in growth performance of weanling pigs. Thus, the results of this study and the current experiment suggest that animal origins do not affect protein quality of blood meals. In conclusion, the current data showed that the two blood meal sources contained high levels of digestible crude protein and most indispensable amino acids. The spray-dried porcine blood meal had greater standardized ileal digestibility of most indispensable amino acids than the drum-dried bovine blood meal. The drying procedure used to produce the two blood meals affected amino acid digestibility, especially lysine. However, both blood meals contained low concentrations of digestible isoleucine, methionine, and cysteine, and thus supplementation of isoleucine and methionine must be considered to increase the feeding value of these blood meals to nursery pigs. Thank you very much for listening, and we hope you can join us again on our next podcast.