Slide 1 Hi. My name is Caroline Gonzalez-Vega, and I'm a Ph. D. student in Dr. Stein's lab. And today I have the pleasure to talk about the effects of using copra meal, palm kernel expellers, or palm kernel meal in diets for weanling pigs. Slide 2 This is the outline of the presentation. First, I will start with a short background about the copra meal and palm kernel products, and then I will mention the materials and methods that were used in these three experiments. And at the end, I will show the results and some conclusions. Slide 3 Copra meal is a coproduct of the coconut oil industry. And in 2011, around 5.9 million metric tons of coconuts were produced in the world. And around 1.9 million metric tons of copra meal was produced. This production is abundant in tropical areas. And copra meal is used mainly as a protein source; however, it is relatively low in lysine and methionine compared with soybean meal. Slide 4 The palm kernel products are coproducts of the palm oil industry. And in 2011, around 13 million metric tons of palm kernels were produced. And around 6.7 million metric tons of palm kernel meal was produced. Palm kernel products are mainly used as a protein source; however they are relatively low in lysine and methionine compared with soybean meal. The palm kernel products that we used in this experiment were palm kernel expellers that are produced after the mechanical oil extraction, and the palm kernel meal that is produced after the solvent oil extraction. Slide 5 This is the analyzed nutrient composition of the copra meal, palm kernel expellers, and palm kernel meal. And as you can see, copra meal and palm kernel expellers contain more gross energy than the palm kernel meal. And palm kernel expellers contains more fat, as acid hydrolyzed ether extract, than the copra meal and the palm kernel meal. However, copra meal contains more crude protein and lysine than the palm kernel products. The palm kernel products contain more insoluble dietary fiber, but the copra meal contains more soluble dietary fiber. Slide 6 And because copra meal contains more soluble dietary fiber, the water binding capacity is also greater than the palm kernel products. The palm kernel expellers contained the greatest bulk density; this may be due to the greater content of fat. Slide 7 Recently, a research conducted in our lab by Sulabo and others were able to determine the digestible energy, metabolizable energy, and the standardized ileal digestibility of amino acids in copra meal, palm kernel expellers from Indonesia and Costa Rica, and palm kernel meal. So as you can see, the copra meal contained the greatest metabolizable energy and digestible lysine and methionine. And among the palm kernel products, the palm kernel meal contained less metabolizable energy and digestible lysine and methionine. Slide 8 Also, in our lab, Almaguer and others were able to determine the standardized total tract digestibility of phosphorus in the same ingredients. And as you can see, copra meal had the greatest digestible phosphorus, and among the palm kernel products, the palm kernel expellers had less digestible phosphorus than the palm kernel meal. It's important to mention that the ingredients that we used for the three experiments were from the same batch that Sulabo and Almaguer used. Therefore, we were able to formulate the diets using the values that were obtained by Sulabo and Almaguer. Also, it's important to mention that we used a mixture of the palm kernel expellers from Indonesia and Costa Rica. Therefore, we used the average values to formulate our diets. Slide 9 The objective of these experiments were to test the hypothesis that copra meal, palm kernel expellers, or palm kernel meal may replace some corn and soybean meal in Phase 2 diets fed to weanling pigs without negatively impacting pig performance. Slide 10 These are the materials and methods that we used. So for the first experiment that we used copra meal, it was conducted in 20 days, and we used 128 pigs with initial body weight of 9.2 kg. And we used four pigs per pen. The second experiment that we used palm kernel expellers, was conducted in 21 days, and we used 128 pigs with initial body weight of 9.8 kg. And we used four pigs per pen. And for the third experiment that we used palm kernel meal, it was conducted in 20 days, and we used 160 pigs with initial body weight of 8.4 kg. We used five pigs per pen. And in each experiment, we had four treatments with 0, 5, 10, and 15% inclusion of the test feed ingredient. Each treatment contains eight replicates. Slide 11 These are the diets for the first experiment containing copra meal. So as you can see, we include 0, 5, 10, and 15% copra meal, replacing some corn and soybean meal. The fish meal was maintaining constant among the diets, and because copra meal contained less digestible energy, then we included more soybean oil to balance the energy for these diets. So as you can see, the metabolizable energy for the diets was maintained constant, and the same for digestible lysine, methionine, and the other amino acids, and also the digestible phosphorus. Slide 12 For Experiment 2 using palm kernel expellers, we used the same procedure as Experiment 1, including 0, 5, 10, and 15% palm kernel expellers. Slide 13 And we did the same with the third experiment. We included 0, 5, 10, and 15% palm kernel meal. Slide 14 We measured the body weight and the feed intake on days 0, 10, and 20 (or 21 for the palm kernel expeller experiment). And we calculated the average daily gain, the average daily feed intake, and gain:feed ratio. Slide 15 We analyzed the data using the Mixed procedure of SAS, and the treatment was the fixed effect, replicate was the random effect, and we used the orthogonal contrast for the linear effects. Slide 16 Now, let's move to the results. But first, let's take a moment to set up the slide. On the x axis, we have the variables – in this case, body weight in kg at day 10 and 20. The yellow bar represents 0% inclusion of the ingredient; the blue bar represents 5% inclusion; the green bar represents 10% inclusion, and the red bar represents 15% inclusion. On top of the bars, we have the P value for the linear effect. So as you can see here, the body weight at day 10 was not affected by the inclusion of copra meal. However, at day 20, the body weight was linearly decreased as we included more copra meal in the diets. Slide 17 The average daily feed intake was linearly decreased as we included more copra meal in the diets. And the possible reason for this observation is that as we included more copra meal in the diets, the water binding capacity also increased, reducing the feed intake. And the average daily gain was also linearly decreased by the inclusion of the copra meal. But this may be due to the reduction of the feed intake. And the gain:feed ratio had a tendency to decrease as we included more copra meal in the diets Slide 18 These are the results for the second experiment using palm kernel expellers. The body weight at day 10 and day 21 were linearly decreased as we included more palm kernel expellers in the diets. Slide 19 There was not an effect of inclusion rate of palm kernel expellers on the average daily feed intake. However, the average daily gain was linearly decreased as more palm kernel expellers were included in the diet. But the gain:feed ratio was not affected by the inclusion of palm kernel expellers. So, in here only the average daily gain was affected by the inclusion of palm kernel expellers. Slide 20 These are the results from the third experiment using palm kernel meal. And as you can see, the body weight at day 10 was linearly decreased by the inclusion of palm kernel meal. But day 20 was not affected by the inclusion of palm kernel meal in the diets. Slide 21 The average daily feed intake and average daily gain were not affected by the inclusion rate of palm kernel meal. However, there was a tendency to decrease the gain:feed ratio when we included more palm kernel meal in the diets. Slide 22 So in conclusion, if Phase 2 diets for weanling pigs are formulated based on metabolizable energy, standardized ileal digestible amino acids, and standardized total tract digestible phosphorus, then at least 15% of palm kernel meal may be included in the diets without affecting overall performance. But if diets contain up to 15% copra meal or palm kernel expellers, then the pig average daily gain will be slightly reduced. Slide 23 And with this, I want to thank you for your attention, and if you want to know more about the monogastric nutrition group, or about other nutrition topics, you can visit our website.