Ileal digestibility of amino acids in low-oil DDGS fed to growing pigs

Distillers dried grains with solubles (DDGS) are fed to pigs as a source of energy and amino acids. In recent years, ethanol plants have begun recovering corn oil from DDGS to sell for biodiesel and other uses. Conventional corn DDGS contains 10-12 fat, compared with 5-9% fat in low oil DDGS. Previous research in our lab showed that amino acid digestibility was reduced in low oil DDGS compared with conventional DDGS, but that research used DDGS from only one supplier. An experiment was conducted to compare the digestibility of amino acids in low oil DDGS sourced from multiple different suppliers.

Experimental design

The experiment used seven sources of DDGS obtained from different suppliers, and these were labeled DDGS A through DDGS H. Diets containing each source of DDGS as the sole source of amino acids were formulated and fed to seven groups of growing barrows (average initial body weight: approximately 65 kg). A nitrogen-free diet was also fed to determine the basal endogenous losses of amino acids and protein.

Ileal digesta samples were collected, and the apparent ileal digestibility (AID) of crude protein and amino acids were calculated for the seven sources of DDGS. The AID of crude protein and amino acids were corrected for basal endogenous losses to determine the standardized ileal digestibility (SID) of these components.

Variability in SID of amino acids among different sources of DDGS

The concentrations of acid hydrolyzed ether extract (AEE) in the seven sources of DDGS ranged from 7.19% in DDGS G to 9.14% in DDGS B (Table 1). There was little variability in the concentrations of crude protein and amino acids among the seven sources.

The SID of crude protein did not differ among the DDGS sources. The SID of lysine ranged from 61.4% in DDGS D to 73.5% in DDGS B (Table 2) and differences among sources were observed (P < 0.05). The SID of most other amino acids also differed (P < 0.05) among sources. The average SID of all amino acids ranged from 78.8% in DDGS D to 88.1% in DDGS B. These differences are likely due to different methods of processing and fat extraction.

The average SID of most AA in the seven sources of DDGS was greater than what has been reported in the past for both low-oil and conventional DDGS. One reason for this observation may be that the concentration of lysine expressed as a percentage of crude protein was greater in all seven of the sources of DDGS used in this work, which indicates that these sources of DDGS were not heat damaged to the same degree as previous sources have been. This likely explains the greater SID of not only lysine but also other amino acids in the DDGS. As a consequence of this increased digestibility of amino acids, the seven sources of DDGS that were included in this work have greater protein value than sources used in previous research.

Key points

  • The amount of fat removed from DDGS varies among suppliers.
  • DDGS sourced from different suppliers varied little in crude protein and amino acid content.
  • There is variability in SID of amino acids among different sources of DDGS, which may be due to different processing methods.
  • The DDGS used in this experiment appears to be less heat damaged compared with DDGS used in previous experiments, which results in increased protein value.

 

Table 1. Analyzed nutrient composition of distillers’ dried grains with solubles (DDGS), as-fed basis

 

Source of DDGS

Item

A

B

C

D

E

G

H

CP, %

27.55

27.82

27.63

27.42

27.90

29.25

28.71

DM, %

88.30

86.70

86.08

87.77

87.46

87.23

89.46

AEE, %

9.10

9.14

7.49

8.70

8.18

7.19

7.94

Indispensable AA, %

   Arg

1.16

1.18

1.20

1.18

1.22

1.25

1.19

   His

0.67

0.72

0.69

0.72

0.71

0.76

0.73

   Ile

1.03

1.09

1.04

1.06

1.01

1.07

1.07

   Leu

2.79

3.13

2.88

3.03

2.83

3.14

2.96

   Lys

0.96

0.95

0.98

0.95

0.96

0.97

1.04

   Met

0.49

0.52

0.50

0.45

0.51

0.52

0.52

   Phe

1.18

1.30

1.21

1.28

1.22

1.31

1.26

   Thr

1.04

1.06

1.05

1.03

1.01

1.12

1.07

   Trp

0.19

0.20

0.20

0.19

0.19

0.19

0.18

   Val

1.41

1.46

1.43

1.41

1.38

1.43

1.44

Dispensable AA, %

   Ala

1.70

1.91

1.79

1.74

1.75

1.94

1.92

   Asp

1.66

1.76

1.69

1.70

1.71

1.78

1.81

   Cys

0.46

0.50

0.47

0.50

0.55

0.53

0.52

   Glu

3.07

3.86

3.36

3.61

3.80

3.82

4.07

   Gly

1.08

1.09

1.08

1.05

1.11

1.11

1.10

   Ser

1.12

1.22

1.17

1.15

1.11

1.30

1.19

   Tyr

0.87

0.93

0.85

0.91

0.86

1.00

0.89

All AA, %

22.95

25.09

23.68

24.28

24.10

25.70

25.20

  Lys:CP, %

3.48

3.41

3.55

3.46

3.44

3.32

3.62

 

Table 2. Standardized ileal digestibility of crude protein and amino acids in distillers’ dried grains with solubles (DDGS)

 

Source of DDGS

 

 

 

Items

A

B

C

D

E

G

H

Mean

SEM

P-value

CP, %

80.4

81.3

81.0

75.5

79.8

82.9

79.7

80.1

1.5

0.089

Indispensable AA, %

   Arg

88.1ab

90.7a

87.6ab

81.5c

89.1ab

87.7ab

86.4b

87.3

1.55

<0.001

   His

81.2ab

82.6a

76.4cd

74.4d

78.3bcd

78.9abc

78.0bcd

78.5

1.40

0.007

   Ile

81.2ab

84.0a

77.7cd

74.6d

79.4bc

79.7bc

78.5bc

79.3

1.26

<0.001

   Leu

87.4ab

89.2a

85.2b

81.9c

81.9c

87.6ab

86.9ab

85.7

0.92

<0.001

   Lys

69.7abc

73.5a

65.4cd

61.4d

70.9ab

66.4bc

66.4bc

67.7

1.72

<0.001

   Met

84.0ab

86.4a

80.8c

77.2d

85.2ab

83.4bc

83.1bc

82.9

1.00

<0.001

   Phe

84.3ab

86.8a

81.6bc

78.8c

83.1b

84.3ab

83.6b

83.2

1.08

<0.001

   Thr

79.1ab

81.9a

75.3bcd

71.4d

76.1bc

74.9cd

71.5d

75.8

1.42

<0.001

   Trp

79.8a

79.9a

74.7ab

73.5b

76.9ab

73.2b

74.2b

76.0

2.54

0.038

   Val

79.6ab

82.4a

76.5bc

73.1d

76.6bc

77.8bc

76.2cd

77.5

1.13

<0.001

 Total

82.6ab

85.1a

79.6b

76.1c

80.3b

81.2b

80.2b

80.7

1.04

<0.001

Dispensable AA, %

   Ala

83.3abc

85.8a

80.4cd

78.4d

80.9bcd

85.2a

84.3ab

82.6

1.21

0.001

   Asp

75.9ab

79.1a

70.3cd

67.0d

71.6bcd

72.7bc

71.4bcd

72.6

1.71

0.001

   Cys

78.2ab

79.4a

69.2cd

66.8d

72.2bcd

74.7abc

72.4bcd

73.3

2.31

0.005

   Glu

84.3ab

86.7a

80.8bc

77.7c

80.4bc

84.5ab

84.3ab

84.1

1.44

0.002

   Gly

80.1

81.6

72.6

70.9

75.5

79.5

76.1

76.6

4.86

0.539

   Ser

83.6ab

86.6a

80.6bc

76.5d

78.3cd

81.1bc

77.8cd

80.6

1.48

<0.001

   Tyr

86.8ab

88.0a

83.4cd

82.0d

84.9bcd

85.6abc

85.4abc

85.2

1.02

0.005

 Total

89.0ab

91.1a

86.1bc

81.5c

85.9bc

88.7ab

87.5ab

87.1

1.63

0.008

All AA

85.7ab

88.1a

82.8b

78.8c

83.1b

85.0ab

83.8b

83.9

1.30

0.001

a-d Means within a row with different superscripts differ (P ≤ 0.05).

This report is based on unpublished research by Charmaine Espinosa and Hans H. Stein.

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