Stein

Why limestone becomes important: Practical implications of supplemental phytase for increased calcium digestibility of limestone in growing pigs

Lee, S. A, and H. H. Stein. 2026. Why limestone becomes important: Practical implications of supplemental phytase for increased calcium digestibility of limestone in growing pigs. Book of abstracts. IFTC 2026. 3rd International Feed Technology Congress. Victam. Utrecht. The Netherlands, June 2-3, 2026. Page 94 - 95 Link to full text.

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Use of feed technology to improve energy and nutrient utilization of diets for growing pigs. Book of abstracts.

Stein, H. H. 2026. Use of feed technology to improve energy and nutrient utilization of diets for growing pigs. Book of abstracts. IFTC 2026. 3rd International Feed Technology Congress. Victam. Utrecht. The Netherlands, June 2-3, 2026. Page 11-14. Link to full text.

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Reducing dietary protein in corn–soybean meal diets by reducing soybean meal and adding synthetic amino acids does not affect net energy or ileal starch digestibility, but increases ileal amino acid digestibility and reduces nitrogen retention

Cristobal, Minoy, Su A. Lee, Carl M. Parsons, Hans H. Stein. 2026. Reducing dietary protein in corn–soybean meal diets by reducing soybean meal and adding synthetic amino acids does not affect net energy or ileal starch digestibility, but increases ileal amino acid digestibility and reduces nitrogen retention. Journal of Animal Science, 2026, 104, skag129 doi.org/10.1093/jas/skag129.

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Soluble dietary fiber, insoluble dietary fiber, and total dietary fiber in feed ingredients used in diets for pigs

Acosta, Jessica P., Lia V. Guardiola, Neil W. Jaworski, Hans H. Stein. 2026. Soluble dietary fiber, insoluble dietary fiber, and total dietary fiber in feed ingredients used in diets for pigs. Journal of Animal Science, 2026, 104, skag141 doi.org/10.1093/jas/skag141 Link to full text.

Reducing dietary crude protein for gestating and lactating sows reduces daily nitrogen retention, but reproductive performance is not impacted by diet protein concentration

Ibagon, Jimena A., Su A Lee, Hans H. Stein. 2026. Reducing dietary crude protein for gestating and lactating sows reduces daily nitrogen retention, but reproductive performance is not impacted by diet protein concentration. Journal of Animal Science and Biotechnology 7:125 

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Digestibility of energy and concentrations of digestible and metabolizable energy in high-protein canola meal, conventional canola meal, and soybean meal in diets fed to lactating sows

Canola meal and soybean meal (SBM) are the most commonly used sources of amino acids in diets for swine. However, via genetic selection, varieties of canola with greater concentrations of crude protein and reduced concentrations of fiber have been identified. Following oil extraction, the resulting high-protein canola meal (CM-HP) contains 3 to 5 % more crude protein than conventional canola meal (CM-CV). It is, therefore, expected that CM-HP contains more digestible and metabolizable energy than CM-CV, but this hypothesis has not been experimentally verified (Liu et al., 2016). Therefore, it is the objective of this experiment to determine the apparent total tract digestibility (ATTD) of energy and concentrations of digestible energy (DE) in CM-HP, CM-CV, and SBM when fed to lactating sows.

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Inclusion of microbial phytase in diets for growing pigs

Jaramillo, B. M., A. V. Hernandez, S. A Lee, M. Cristobal, and H. H. Stein. 2026. Inclusion of microbial phytase in diets for growing pigs. National Hog Farmer. On-line edition. April 30, 2026. Link to full text.

Effects of graded levels of phytase on apparent total tract digestibility of energy and concentrations of digestible and metabolizable energy in soybean meal

Soybean meal (SBM) is commonly used as a source of amino acids in swine diets because of its high protein content (Kudelka et al., 2021).  In addition, SBM contributes with energy and phosphorus (P). However, high portion of the P in SBM is bound to phytate, which is indigestible by pigs. Therefore, microbial phytase enzyme is supplemented in swine diets. The impact of phytase on the digestibility of P is well documented, however some experiments on digestibility in corn–SBM diets has been reported energy increase if microbial phytase was used (Liao et al., 2005; Arredondo et al., 2019; Lala et al., 2020; Espinosa et al., 2022), but, not in others (She et al., 2018; Mesina et al., 2019; Lamp and Moritz, 2022). There is, however, a lack of information about the impact of microbial phytase on the digestibility of energy in SBM. It is also not known if a possible effect of microbial phytase on energy digestibility in SBM is consistent among different sources of SBM.

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Late-Breaking: Gestation stage, lactation, and partial substitution of hydroxy minerals with methionine hydroxy analogue chelated minerals impacts nutrient retention.

Acosta, Jesus A., Brad V. Lawrence, Eric Myers, Chelsie K. K. Foran, Hans H. Stein, Deana Hancock. 2026. Late-Breaking: Gestation stage, lactation, and partial substitution of hydroxy minerals with methionine hydroxy analogue chelated minerals impacts nutrient retention. Journal of Animal Science, Volume 103, Issue Supplement_3, October 2025, Pages 191–192, doi.org/10.1093/jas/skaf300.230 Link to the abstract.

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Determination of digestible indispensable amino acid score for salmon hydrolysate proteins

Fanelli, Natalia S., Juliana C.F.R. Martins, Hans H. Stein. 2026. Determination of digestible indispensable amino acid score for salmon hydrolysate proteins. Journal of the Science of Food and Agriculture. DOI 10.1002/jsfa.70518 Link to full text.

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Inclusion of pistachio blanks in diets for weanling pigs has limited impact on growth performance and blood characteristics

Kim, Yeonwoo, Hans H. Stein. 2026. Inclusion of pistachio blanks in diets for weanling pigs has limited impact on growth performance and blood characteristics. Animal Feed Science and Technology 338 (2026) 116746. doi.org/10.1016/j.anifeedsci.2026.116746 Link to full text.

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The concentration of energy in urine and the concentration of metabolizable energy in diets fed to pigs are influenced by urine preparation and drying method

Ibagon, J.A.,  L.V. Lagos, H.H. Stein. 2026. The concentration of energy in urine and the concentration of metabolizable energy in diets fed to pigs are influenced by urine preparation and drying method. Animal Feed Science and Technology 339 (2026) 116778. doi.org/10.1016/j.anifeedsci.2026.116778 Link to full text.

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Digestibility of Energy and Concentrations of Digestible and Metabolizable Energy in Pistachio Shell Powder and in Soybean Hulls Fed to Growing Pigs

Kim, Yeonwoo, Maryane S. F. Oliveira, Su A Lee, Hans H. Stein. 2026. Digestibility of Energy and Concentrations of Digestible and Metabolizable Energy in Pistachio Shell Powder and in Soybean Hulls Fed to Growing Pigs. Animals Animals 2026, 16, 758. doi.org/10.3390/ani16050758 Link to full text.

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Digestibility of energy and concentrations of digestible and metabolizable energy in high-protein canola meal, conventional canola meal, and soybean meal in diets fed to gestating sows

Canola meal and soybean meal (SBM) are the most commonly used sources of amino acids in diets for swine. However, via genetic selection, varieties of canola with greater concentrations of crude protein and reduced concentrations of fiber have been identified. Following oil extraction, the resulting high-protein canola meal (CM-HP) contains 3 to 5 % more crude protein than conventional canola meal (CM-CV). It is, therefore, expected that CM-HP contains more digestible and metabolizable energy than CM-CV when fed to sows, but this hypothesis has not been experimentally verified (Liu et al., 2016). Therefore, it was the objective of this experiment to determine the apparent total tract digestibility (ATTD) of energy and concentrations of digestible energy (DE) and metabolizable energy (ME) in high protein canola meal, conventional canola meal, and SBM when fed to gestating sows.

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Pistachio shell powder may be included in diets for weanling pigs without compromising growth performance or blood characteristics

Kim, Yeonwoo, Su A Lee, and Hans H. Stein. 2026. Pistachio shell powder may be included in diets for weanling pigs without compromising growth performance or blood characteristics. Translational Animal Science, 2026, 10, txag002. doi.org/10.1093/tas/txag002 Link to full text.

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Digestibility of energy and concentrations of digestible and metabolizable energy in high-protein canola meal, conventional canola meal, and soybean meal in diets fed to growing pigs

Canola meal and soybean meal are the most commonly used amino acid sources in diets for growing pigs. However, through genetic selection, varieties of canola with greater concentrations of crude protein and reduced concentrations of fiber have been identified. Following oil extraction, the resulting high-protein canola meal contains 3 to 5 percent more crude protein than conventional canola meal. It is, therefore, expected that high-protein canola meal contains more digestible energy (DE) and metabolizable energy (ME) than conventional canola meal when fed to growing pigs. Therefore, experiment was conducted to test the hypothesis that DE and ME in high-protein canola meal is greater than in conventional canola meal.

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Effects of a novel microbial phytase on digestibility of P and Ca in diets fed to young growing pigs

Introduction

Pigs have a poor utilization of phytate-bound P derived from plant feed ingredients and supplemental phytase is, therefore, often used in pig diets (Selle and Ravindran, 2008), which is a very effective way to increase P digestibility. However, digestibility of Ca is also increased by phytase (Lee et al., 2023).

Bestzyme has recently been developed as a novel phytase, but there is limited information on how this phytase works on P and Ca digestibility. Therefore, the objective of this experiment was to test the hypothesis that a novel microbial phytase can replace some of the dietary P and Ca in diets for growing pigs without negatively impacting the standardized total tract digestibility (STTD) of P and Ca.

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