Hans H. Stein

microbial phytase, Growth performance, Bone ash, Growing pigs

Effects of novel microbial phytases on growth performance and bone ash of young growing pigs

Pigs have a poor utilization of phytate-bound P derived from plant feed ingredients and thus supplemental phytase is used in pig diets (Selle and Ravindran, 2008). Use of phytase in diets has been a very effective way to increase P digestibility, but the digestibility of amino acids, energy, and other minerals may also be increased by super dosing phytase (Kies et al., 2006; Arredondo et al., 2019), which ultimately increases growth performance and bone ash of pigs.

Bestzyme has recently been developed as a novel phytase, but there is limited information on how this phytase works on growth performance and bone ash of pigs. Therefore, the objective of this experiment was to test the hypothesis that dietary P and Ca in diets for growing pigs can be reduced without negatively impacting growth performance and bone ash of pigs if Bestzyme phytase is included in the diets.

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Authors:

Mallea, Stein

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Effect of feed conditioning time prior to pelleting on standardized ileal digestibility of amino acids and total tract digestibility of energy in diets fed to growing pigs

Lopez, Diego A., Hans H. Stein, Matt D. Miesner, Jordan T. Gebhardt, Charles R. Stark, and Chad B. Paulk. 2025. Effect of feed conditioning time prior to pelleting on standardized ileal digestibility of amino acids and total tract digestibility of energy in diets fed to growing pigs. Translational Animal Science, 2025, 9, txaf153

Keywords:

Amino acids, conditioning, Digestibility, Energy, Pelleting, Pigs

Authors:

Lopez, Stein, Miesner, Gebhardt, Stark, Paulk

Publication Type:

Growth performance, Weanling pigs, acidifier

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Growth performance of weanling pigs fed diets supplemented with a novel acidifier

The post-weaning period is a critical period for pigs and is often associated with reduced feed intake, impaired gut health, and reduced growth performance. Pharmacological concentrations of zinc oxide have been widely used in nursery diets to alleviate these challenges, but increasing environmental and regulatory concerns have encouraged the evaluation of alternative nutritional strategies. Dietary acidifiers have been shown to reduce gastrointestinal pH, limit the proliferation of pathogenic bacteria, and support nutrient utilization in young pigs. As a result, novel acidifiers may represent a practical alternative to zinc oxide in diets for weanling pigs. Therefore, the objective of this study was to test the hypothesis that the addition of the acidifier Protacid Soy Plus (PSP; Higienizo, Madrid, Spain) to diets for weanling pigs would increase growth performance.

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Authors:

Mallea, Ibagon, Stein

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pig, true ileal amino acid digestibility, Amino acids, protein quality, Protein digestibility

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True Ileal Amino Acid Digestibility of Human Foods Classified According to Food Type as Determined in the Growing Pig

Hodgkinson, Suzanne M., Natascha Stroebinger, Hans H. Stein, Natalia S. Fanelli, Sonja de Vries, Nikkie van der Wielen, Wouter H. Hendriks, Paul J Moughan.

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Authors:

Hodgkinson, Stroebinger, Stein, Fanelli, de Vries, van der Wielen, Hendriks, Moughan

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Effects of the combination of spray dried plasma and reduced crude protein in diets on growth performance, diarrhea scores, gut morphology, and immune parameters of weanling pigs

Bailey, Hannah M., Natalia S. Fanelli, Joy M. Campbell, and Hans H. Stein. 2025. Effects of the combination of spray dried plasma and reduced crude protein in diets on growth performance, diarrhea scores, gut morphology, and immune parameters of weanling pigs. Can. J. Anim. Sci.

Keywords:

cytokines, inflammation, Low crude protein, Spray Dried Plasma, Weanling pigs

Authors:

Bailey, Fanelli, Campbell, Stein

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Feeding intact protein from soybean meal instead of corn and synthetic amino acids does not affect growth performance, carcass composition, blood cytokines, or mRNA abundance of intestinal amino acid transporters in growing pigs, but net energy tends to b

Cristobal, Minoy, Su A Lee , Carl M. Parsons, and Hans H. Stein.

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Amino acids, Carcass composition, Net energy, Pigs, Protein, Soybean meal

Authors:

Cristobal, Lee, Parsons, Stein

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Read more about Feeding intact protein from soybean meal instead of corn and synthetic amino acids does not affect growth performance, carcass composition, blood cytokines, or mRNA abundance of intestinal amino acid transporters in growing pigs, but net energy tends to b

An expert consensus framework for a database on ileal amino acid digestibility and protein quality scoring from foods consumed by humans.

Tome, D., J. Calvez, R. Elango,.E. Ferriolli, C. Gaudichon, G. Courtney-Martin, F. Han, M. Hayes, S. Hodgkinson, A. Kurpad, J. J. Mes, V. Owino, I. Recio, S. P. Shertukdef, H.H. Stein, A. Vlassopoulos, M. Xipsiti. 2025. An expert consensus framework for a database on ileal amino acid digestibility and protein quality scoring from foods consumed by humans. Current Dev. Nutr. 9:107291. doi.org/10.1016/j.cdnut.2025.107291 Link to abstract.

Keywords:

ileal, Amino Acid, Digestibility

Authors:

Tome, Calvez, Elango, Ferriolli, Gaudichon, Courtney-Martin, Han, Hayes, Hodgkinson, Kurpad, Mes, Owino, Recio, Shertukdef, Stein, Vlassopoulos, Xipsiti.

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Salmon, protein hydrolystes Amino acids

Protein quality of salmon protein hydrolystes using the digestible indispensable amino acid score (DIAAS) method

Fanelli, N. S., J. C. F. R. Martins, and H. H. Stein. 2025. Protein quality of salmon protein hydrolystes using the digestible indispensable amino acid score (DIAAS) method. Current Dev. Nutr. 9:106016. doi.org/10.1016/j.cdnut.2025.106593 Link to abstract.

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Authors:

Fanelli, Martins, Stein

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Distillers dried grains with solubles, Net energy, nitrogen retention, Pigs, Starch

Determination of net energy and nitrogen balance in group-housed pigs fed diets containing varying levels of starch and fiber

Lee, S. A, and H. H. Stein. 2025. Determination of net energy and nitrogen balance in group-housed pigs fed diets containing varying levels of starch and fiber. J. Anim. Sci. 103(Supplement_3), 181-182. doi.org/10.1093/jas/skaf300.221 Link to abstract.

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Authors:

Lee, Stein

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Greenhouse gas emission, nitrogen balance, and ileal digestibility of amino acids and acid hydrolyzed ether extract in diets containing different inclusion rates of hybrid rye fed to growing pigs

Acosta, J. P., S. A Lee, and H. H. Stein. 2025. Greenhouse gas emission, nitrogen balance, and ileal digestibility of amino acids and acid hydrolyzed ether extract in diets containing different inclusion rates of hybrid rye fed to growing pigs. J. Anim. Sci. 103(Suppl3), 186-187. doi.org/10.1093/jas/skaf300.224 Link to abstract.

Keywords:

Digestibility, greenhouse gas emission, Hybrid rye, Pigs

Authors:

Acosta, Lee, Stein

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Extension Reports and other non-refereed publications

Soybean meal remains a valuable pig feed ingredient

Cristobal, M., S. A. Lee, C. M. Parsons, and H. H. Stein. 2025. Soybean meal remains a valuable pig feed ingredient. National Hog Farmer, On-line edition, October 30, 2025. Link to full text.

Keywords:

Soybean meal, pig, feed ingredient

Authors:

Cristobal, Lee, Parsons, Stein

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Effects of heat damage and crystalline amino acid supplementation on growth performance of weanling pigs fed soybean meal and enzyme-treated soybean meal

Excessive heat during processing can reduce the concentration and bioavailability of Lys in soybean ingredients via Maillard reactions, lowering growth performance in weanling pigs. Reactive Lys (the Lys not bound in Maillard products) is the relevant bioavailable part of Lys that can be used for protein synthesis by the animals. We tested the hypothesis that: (1) growth performance can be predicted from reactive Lys supplied by heat-damaged soybean ingredients, and (2) crystalline amino acids (AA) can be added to the diet to compensate for heat-induced losses.

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Enzyme-treated soybean meal, Growth performance, Maillard reaction, reactive Lys, Weanling pigs

Authors:

Torres-Mendoza, Stein

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Choice white grease equivalence of fat emulsifier in diets fed to growing pigs

Lee, S. A., V. Perez, H. Stein. 2025. Choice white grease equivalence of fat emulsifier in diets fed to growing pigs. Animal - Science proceedings, Volume 16, Issue 2, August 2025, Page 384 doi.org/10.1016/j.anscip.2025.07.166 Link to abstract.

Keywords:

Emulsifier, Equivalence, Fat

Authors:

Lee, Pérez, Stein

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Corn, pistachio shell powder, Weanling pigs

Effect of pistachio shell powder on growth performance of weanling pigs

Kim, Y. S. A. Lee, H. Stein. 2025. Effect of pistachio shell powder on growth performance of weanling pigs. Animal - Science proceedings, Volume 16, Issue 2, August 2025, Page 380. doi.org/10.1016/j.anscip.2025.07.156 Link to abstract.

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Authors:

Kim, Lee, Stein

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Effects of supplemental phytase on growth performance, blood inositol levels, and immune characteristics of weanling pigs

Mallea, A., S.A. Lee, H. Stein. 2025. Effects of supplemental phytase on growth performance, blood inositol levels, and immune characteristics of weanling pigs. Animal - Science proceedings, Volume 16, Issue 2, August 2025, Pages 374-375 doi: 10.1016/j.anscip.2025.07.144 Link to abstract.

Keywords:

inositol, Phytase, Weanling pigs.

Authors:

Mallea, Lee, Stein

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Effects of dietary protein on nitrogen balance, reproductive performance, colostrum and milk compositions, and blood immune characteristics of sows

Soybean meal (SBM) is an important protein source in swine diets, but inclusion is sometimes reduced and SBM is partially replaced with synthetic amino acids (AA) because this may sometimes reduce diet costs. Over the past few decades, reproductive efficiency in sows has improved significantly, due to genetic selection, resulting in litters that can exceed 20 piglets. This increase in prolificacy has, therefore, increased the metabolic demands for AA and energy during both gestation and lactation. Adequate energy intake in gestation supports fetal growth and mammary development, but excessive energy intake during gestation may reduce feed intake during lactation, which has a negative effect on milk production. Likewise, high protein intake during gestation will improve milk production, protein accretion, and litter and pig weight at weaning, but it may also lead to an increase in fat accretion rather than protein accretion in gestating sows.

Keywords:

Amino acids, nitrogen balance, nitrogen retention, Pigs, soybean meal.

Authors:

Ibagon, Stein, Lee

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Novel milk ingredient blend in nursery pig diets did not improve growth performance and survival compared to control diets without or with spray-dried plasma

Shen, Yanbin, Sung Woo Kim, Hans H. Stein, Javier Polo, and Joe Crenshaw. 2025. Novel milk ingredient blend in nursery pig diets did not improve growth performance and survival compared to control diets without or with spray-dried plasma. Front. Anim. Sci. 6:1648283.

Keywords:

weaned pigs, milk protein, spray-dried plasma, diarrhea score, pig survival

Authors:

Shen, Kim, Stein, Polo, Crenshaw

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