Determination of amino acid digestibility in soybean meal from different regions of the United States and fed to pigs

Soybeans grown in the northern United States are exposed to fewer growing days and hours of sunlight than soybeans grown elsewhere in the U.S. As a result, soybeans grown in the northern U.S. fix less nitrogen, and have a lower concentration of crude protein, than other U.S. soybeans. However, the concentrations of particular amino acids, particularly indispensable amino acids, are more important for the purposes of diet formulation than the concentration of crude protein. The concentration of amino acids in soybeans grown in different parts of the U.S. has not been determined.

The amount of amino acids in soybean meal that are available to the pig also depends on digestibility, but no research has been conducted to compare the digestibility of amino acids among soybean meal produced in different regions of the U.S. Therefore, an experiment was conducted to compare the standardized ileal digestibility (SID) of amino acids and the concentration of SID amino acids in soybean meal produced in different regions within the United States and fed to growing pigs.

Materials and methods

Twenty sources of soybean meal were used in the experiment. Soybeans were sourced from crushing plants located throughout the soybean growing area of the U.S. For analysis, the crushing plant locations were separated into 4 zones: 1) northern growing area (Michigan, Minnesota, and South Dakota; 4 samples), 2) eastern growing area (Georgia, Indiana, and Ohio; 6 samples), 3) western growing area (Iowa, Missouri, and Nebraska; 7 samples), and 4) Illinois (5 samples).

Twenty-two growing barrows with an average initial body weight of 25.5 kg were fed one of 22 diets. The diets were based on cornstarch, sucrose, and soybean meal from each of the four zones. Soybean meal was the only source of amino acids in the diets. Ileal samples were collected and analyzed to determine ileal digestibility of crude protein and amino acids.

Results

The SID of crude protein was greater (P < 0.05) in soybean meal from Zones 1 and 3 than in soybean meal from Zone 4, and the same as in soybean meal from Zone 2 (Table 1). Minor differences in the SID of some individual amino acids were observed, but there was no difference in the average SID of indispensable amino acids. The average SID of all amino acids was greatest (P < 0.05) in soybean meal from Zone 3 and least in soybean meal from Zone 2, but the difference was only 1.07 percentage points.

The concentration of SID crude protein was greater (P < 0.05) in soybean meal from Zone 2 than in soybean meal from Zone 3, but not different than in soybean meal from Zones 1 and 4 (Table 2). With the exception of minor differences in threonine and tyrosine concentrations, there was no difference in the amount of SID amino acids among soybean meal from the different zones.

Key points

  • There were no differences in the average SID of indispensable amino acids, and only minor differences in the average SID of total amino acids, among soybean meal sourced from four different zones in the United States. 
  • There were no differences in the concentration of SID indispensable amino acids, SID dispensable amino acids, or SID total amino acids among soybean meal from the four different zones.
  • The growing area of U.S. soybeans does not influence the protein value of soybean meal fed to pigs.

Table 1. Standardized ileal digestibility (%) of crude protein and amino acids in soybean meal by growing pigs

 

Zones1

 

 

 

Item, %

1

2

3

4

Average

SEM

P-value

CP

95.69a

94.85ab

94.98a

93.59b

94.78

0.60

0.04

Indispensable AA

 

 

 

 

 

 

Arg

98.17

97.98

98.27

97.83

98.06

0.31

0.22

His

95.02ab

94.10b

95.09a

94.14b

94.59

0.36

0.04

Ile

93.77ab

93.07b

94.05a

93.18ab

93.52

0.39

0.03

Leu

93.49ab

92.66ab

93.74a

92.97b

93.22

0.40

0.02

Lys

93.75a

92.51bc

93.36ab

92.27c

92.97

0.45

0.03

Met

95.07

94.76

95.38

94.65

94.96

0.39

0.12

Phe

93.79ab

92.91b

93.98a

93.23ab

93.48

0.38

0.01

Thr

92.58

91.37

92.67

91.78

92.10

0.62

0.06

Trp

95.95

95.36

95.88

95.07

95.56

0.43

0.10

Val

93.54ab

92.62b

93.83a

92.65b

93.16

0.51

0.04

Mean

89.76

89.18

90.06

89.21

89.55

0.41

0.08

Dispensable AA

 

 

 

 

 

 

Ala

93.21ab

91.90b

93.62a

92.45ab

92.80

0.65

0.02

Asp

92.39a

91.58ab

92.52a

90.83b

91.83

0.50

0.02

Cys

91.17a

89.79ab

91.48a

89.15b

90.40

0.75

0.05

Glu

93.65

92.79

93.63

92.99

93.27

0.59

0.18

Ser

96.01

95.39

96.01

95.40

94.95

0.43

0.20

Tyr

93.43ab

92.60b

93.68a

92.98ab

93.17

0.39

0.02

Mean

90.12ab

89.36b

90.58a

89.51ab

89.97

0.52

0.04

All AA

89.28ab

88.62b

89.69a

88.73ab

89.08

0.45

0.05

a-eMeans within a row lacking a common superscript letter are different (P < 0.05).

1Zone = 1: northern growing area (MI, MN, and SD); Zone 2 = eastern growing area (GA, IN, and OH); Zone 3 = western growing area (IA, MO, and NE); Zone 4 = Illinois.

Table 2. Concentrations (g/kg) of standardized ileal digestible crude protein and amino in soybean meal

 

Zones1

 

 

 

Item, g/kg

1

2

3

4

Average

SEM

P-value

CP

446.30ab

459.47a

441.61b

449.78ab

449.30

6.12

0.03

Indispensable AA

 

 

 

 

 

 

Arg

33.01

33.77

33.12

33.69

33.40

0.43

0.25

His

11.24

11.48

11.33

11.54

11.40

0.15

0.21

Ile

19.17

20.09

19.83

20.26

19.84

0.53

0.20

Leu

33.42

33.87

33.36

34.34

33.75

0.41

0.09

Lys

28.08

28.48

27.90

28.11

28.14

0.41

0.28

Met

6.06

6.00

5.95

6.08

6.02

0.08

0.29

Phe

21.22

21.65

21.25

21.85

21.49

0.32

0.21

Thr

16.20ab

16.39ab

16.06b

16.61a

16.31

0.17

0.03

Trp

6.69

6.72

6.66

6.75

6.70

0.08

0.51

Val

20.32

21.21

21.06

21.31

20.97

0.57

0.27

Total

195.41

199.66

196.62

200.54

198.02

2.67

0.22

Dispensable AA

 

 

 

 

 

 

Ala

18.27

18.37

18.31

18.69

18.41

0.19

0.14

Asp

47.03

47.61

46.88

47.32

47.21

0.56

0.32

Cys

5.67

5.65

5.62

5.67

5.65

0.09

0.70

Glu

72.20

72.29

71.87

72.48

72.21

1.06

0.68

Ser

20.64

20.50

19.99

20.68

20.45

0.36

0.17

Tyr

15.42b

15.70ab

15.52b

16.05a

15.67

0.16

0.02

Total

179.23

180.12

178.19

180.89

179.61

2.22

0.30

All AA

374.64

379.78

374.81

381.43

377.63

4.62

0.26

a-bMeans within a row lacking a common superscript letter are different (P < 0.05).

1Zone = 1: northern growing area (MI, MN, and SD); Zone 2 = eastern growing area (GA, IN, and OH); Zone 3 = western growing area (IA, MO, and NE); Zone 4 = Illinois.

 

This report is based on research by Kelly Sotak-Peper and Hans H. Stein, published in Sotak-Peper's Ph. D. dissertation.

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