ileal digesta

Addition of hydrochloric acid to collection bags or collection containers did not change basal endogenous losses or ileal digestibility of amino acid in corn, soybean meal, or wheat middlings fed to growing pigs

Lee, Su A, Laia Blavi, Diego M. D. L. Navarro, and Hans H. Stein. 2021. Addition of hydrochloric acid to collection bags or collection containers did not change basal endogenous losses or ileal digestibility of amino acid in corn, soybean meal, or wheat middlings fed to growing pigs. Animal Bioscience Vol. 34, No. 10:1632-1642 October 2021. doi.org/10.5713/ab.20.0838.

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Effects of adding HCl to collection bags or collection pitchers on amino acid digestibility in corn, soybean meal, or wheat middlings fed to growing pigs

In most AA digestibility experiments collection bags are changed at least every 30 min and the collected ileal digesta are stored at –20 °C to prevent microbial degradation of proteins. Other attempts to reduce microbial activity in digesta include adding acids to collection bags to reduce the pH in ileal digesta. Acids may also be added to collection pitchers. However, in some experiments, no attempt to reduce pH is made because it is assumed that the rapid reduction in temperature after collection is sufficient to prevent microbial degradation of proteins.

To our knowledge, there is, however, no information about the necessity of adding acids to collected digesta and it is not known if values for apparent ileal digestibility (AID), basal endogenous losses of AA, or standardized ileal digestibility (SID) of AA are influenced by addition of acids to digesta. Therefore, the objective of this experiment was to test the null-hypothesis that values for AID, basal endogenous losses, and SID of AA are not influenced by addition of acids to ileal digesta collection bags or to collection pitchers.

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Oven drying of ileal digesta from growing pigs reduces the concentration of AA compared with freeze drying and results in reduced calculated values for endogenous losses and elevated estimates for ileal digestibility of AA

Lagos L. Vanessa, and Hans H. Stein. 2019. Oven drying of ileal digesta from growing pigs reduces the concentration of AA compared with freeze drying and results in reduced calculated values for endogenous losses and elevated estimates for ileal digestibility of AA. J. Anim. Sci. 2019.97:820–828. Link to full text.

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