Amino acids

Digestibility of amino acids in a high protein corn product fed to growing pigs

Corn coproducts produced from the ethanol industry are often used in diets for pigs. A new source of corn protein (NexPro; Flint Hills Resources, Wichita, KS) has been developed, but at this time there is limited information about the nutritional value of this new source of corn protein. Therefore, the objective of this experiment was to test the hypothesis that the standardized ileal digestibility (SID) of amino acids  in corn protein is greater than in 2 sources of distillers dried grains with solubles (DDGS; i.e., DDGS-1 and DDGS-2) when fed to growing pigs.

Publication Type: 

Conditioning and expansion increase nutritional value of soybean expellers

Soybean expellers contain trypsin inhibitors, which negatively affect nutrient digestibility, feed efficiency, and health status of animals. Therefore, heat treatment is needed to inactivate trypsin inhibitors to improve nutrient digestibility in soybean expellers. Different types and degrees of processing conditions may influence digestibility of energy and amino acids (AA) in soybean expellers, but there is a lack of data demonstrating how long heat treatment is needed if soybean expellers are expander processed after hydrothermical conditioning. Therefore, 2 experiments were conducted to test the hypothesis that the standardized ileal digestibility (SID) of AA, as well as values for digestible energy (DE) and metabolizable energy (ME) in soybean expellers increase the longer heat is applied to the expellers.

Authors: 
Publication Type: 

Standardized ileal digestibility of amino acids in six sources of partially de-hulled sunflower meal and one source of sunflower expellers from Ukraine, Hungary, Italy, and the U.S. when fed to growing pigs

Sunflower meal, which is the co-product derived from sunflower seeds after oil extraction, has a high concentration of digestible amino acids (AA) and fiber. The nutritive value and quality of sunflower meal is partially dependent on the degree of dehulling, the variety of the sunflower, and the oil extraction process. Sunflower seeds are initially de-hulled and then partially de-oiled using a mechanial prepress procedure. A second de-oiling procedure is performed using a solvent extraction procedure or by usign a second mechanical expelling procedure. The prepress-solvent extraction procedure results in production of sunflower oil and sunflower meal that contains 1 to 3% oil, whereas the double-press procedure results in production of sunflower oil and a sunflower product that contains 5 to 10% residual oil and commonly is referred to as sunflower expellers. Some of the hulls may be added back to the sunflower meal or the sunflower expellers resulting in differences in concentrations of total dietary fiber among sources.

The apparent ileal digestibility and the standardized ileal digestibility (SID) of crude protein (CP) and AA in individual sources of partially de-hulled sunflower meal fed to pigs have been reported. However, there are no comparative values for the SID of AA in sunflower meal produced in different parts of the world, and it is not known if the SID of AA in sunflower meal are different from those in sunflower expellers. Therefore, the objective of the current study was to test the hypothesis that there is no difference in the SID of CP and AA in different sources of sunflower meal and sunflower expellers obtained from different countries.

Authors: 
Publication Type: 

Swine Nutrition

Stein, H. H. 2020. Swine Nutrition. Feedstuffs Reference Issue & Nutritional Guide. P. 35-40. Link to full text

Authors: 

Nutrition Know-How: High-Shear Dry Soybean Expellers

Rodriguez, D. A., S. A Lee, and H. H. Stein. 2020. Nutrition Know-How: High-Shear Dry Soybean Expellers. Pork Magazine, On-line edition, Aug. 2020. Link to full text.

Authors: 

Digestibility of amino acids, but not fiber, fat, or energy, is greater in cold-fermented, low-oil distillers dried grains with solubles (DDGS) compared with conventional DDGS fed to growing pigs

Rodriguez, Diego A., Su A Lee, and Hans H. Stein. 2020. Digestibility of amino acids, but not fiber, fat, or energy, is greater in cold-fermented, low-oil distillers dried grains with solubles (DDGS) compared with conventional DDGS fed to growing pigs. Journal of Animal Science, 2020, Vol. 98, No. 10, 1–8. doi:10.1093/jas/skaa297.

Authors: 

Effects of adding HCl to collection bags or collection pitchers on amino acid digestibility in corn, soybean meal, or wheat middlings fed to growing pigs

In most AA digestibility experiments collection bags are changed at least every 30 min and the collected ileal digesta are stored at –20 °C to prevent microbial degradation of proteins. Other attempts to reduce microbial activity in digesta include adding acids to collection bags to reduce the pH in ileal digesta. Acids may also be added to collection pitchers. However, in some experiments, no attempt to reduce pH is made because it is assumed that the rapid reduction in temperature after collection is sufficient to prevent microbial degradation of proteins.

To our knowledge, there is, however, no information about the necessity of adding acids to collected digesta and it is not known if values for apparent ileal digestibility (AID), basal endogenous losses of AA, or standardized ileal digestibility (SID) of AA are influenced by addition of acids to digesta. Therefore, the objective of this experiment was to test the null-hypothesis that values for AID, basal endogenous losses, and SID of AA are not influenced by addition of acids to ileal digesta collection bags or to collection pitchers.

Authors: 
Publication Type: 

The direct and difference procedures result in similar estimates for amino acid digestibility in feed ingredients fed to growing pigs

Oliveira, Maryane S. F., John K. Htoo, and Hans H. Stein. 2020. The direct and difference procedures result in similar estimates for amino acid digestibility in feed ingredients fed to growing pigs. Journal of Animal Science, 2020, Vol. 98, No. 8, 1–8. doi:10.1093/jas/skaa225. Link to full text.

Authors: 

Values for digestible indispensable amino acid score (DIAAS) determined in pigs for breakfast cereals and milk are additive in combined breakfast cereal-milk meals

Breakfast cereals are usually consumed with milk as a breakfast meal. To meet requirements for amino acids (AA), higher quality proteins are needed to complement the protein in cereals to provide a meal that is adequate in all indispensable AA. The digestible indispensable amino acid score (DIAAS) method used to determine protein quality allows for calculation of the protein value of individual ingredients and mixed meals consisting of several proteins. Values for DIAAS are based on values for apparent ileal digestibility (AID) of AA that are corrected for the basal endogenous loss of each individual AA, resulting in values defined as standardized ileal digestibility (SID). Values for SID of AA are additive in mixed meals because these values are independent of basal endogenous losses. Consequently, it is expected that DIAAS obtained for individual food ingredients are additive in a mixed meal, but data to demonstrate this have not been reported. Therefore, the objective of this experiment was to test the hypothesis that AA in milk complement AA in breakfast cereals to provide a balanced meal and that DIAAS in individual foods are additive in a combined meal.  

Authors: 
Publication Type: 

Determination of true ileal amino acid digestibility in the growing pig for calculation of digestible indispensable amino acid score (DIAAS)

Hodgkinson, Suzanne M., Hans H. Stein, Sonja de Vries, Wouter H. Hendriks, and Paul J. Moughan. 2020. Determination of true ileal amino acid digestibility in the growing pig for calculation of digestible indispensable amino acid score (DIAAS). The Journal of Nutrition. First published online, 2020. doi.org/10.1093/jn/nxaa210.

A new source of high-protein distillers dried grains with solubles (DDGS) has greater digestibility of amino acids and energy, but less digestibility of phosphorus, than de-oiled DDGS when fed to growing pigs

Cristobal, Minoy, Jessica P. Acosta, Su A Lee, and Hans H. Stein. 2020. A new source of high-protein distillers dried grains with solubles (DDGS) has greater digestibility of amino acids and energy, but less digestibility of phosphorus, than de-oiled DDGS when fed to growing pigs. Journal of Animal Science, 2020, Vol. 98, No. 7, 1–9. doi:10.1093/jas/skaa200.

Authors: 

Effects of isoquinoline alkaloids on apparent ileal digestibility of amino acids, acid hydrolyzed ether extract, and starch by young growing pigs fed corn-soybean meal diets

Rundle, Carly M., Valeria Artuso-Ponte, and Hans H. Stein. 2020. Effects of isoquinoline alkaloids on apparent ileal digestibility of amino acids, acid hydrolyzed ether extract, and starch by young growing pigs fed corn-soybean meal diets. Transl. Anim. Sci. 2020.4:1-7. doi: 10.1093/tas/txaa054. Link to full text. 

Most meat products have digestible indispensable amino acid scores that are greater than 100, but processing may increase or reduce protein quality

Bailey, Hannah M., John K. Mathai, Eric P. Berg, and Hans H. Stein. 2020. Most meat products have digestible indispensable amino acid scores that are greater than 100, but processing may increase or reduce protein quality. British Journal of Nutrition (2020), 124, 14–22. doi:10.1017/S0007114520000641. Link to full text.

Authors: 

Impact of increasing the levels of insoluble fiber and on the method of diet formulation measures of energy and nutrient digestibility in growing pigs

Acosta, Jesus A., Hans H. Stein, and John F. Patience. 2020. Impact of increasing the levels of insoluble fiber and on the method of diet formulation measures of energy and nutrient digestibility in growing pigs. Journal of Animal Science, 2020, 1–9. doi:10.1093/jas/skaa130. Link to full text.

Authors: 

Torula yeast has greater digestibility of amino acids and phosphorus, but not energy, compared with a commercial source of fish meal fed to weanling pigs

Lagos, Vanessa L., Hans H. Stein. 2020. Torula yeast has greater digestibility of amino acids and phosphorus, but not energy, compared with a commercial source of fish meal fed to weanling pigs. Journal of Animal Science, 2020, 1-9. doi:10.1093/jas/skz375. Link to full text.

Authors: 

Effects of super dosing 4 different sources of phytase on amino acid digestibility

Dietary phytate may bind to proteins from feed ingredients by making indigestible nutrient-complexes. Therefore, it is possible that adding exogenous phytase to the diets increases digestibility of amino acids (AA). However, results of experiments in which microbial phytase has been added to diets fed to pigs have not consistently demonstrated increased ileal digestibility of AA. It is, however, possible that is because the dose of phytase was too low to obtain a positive effect on AA digestibility and that if greater doses were used, a positive response would be obtained. Therefore, the objective of this experiment was to test the hypothesis that super dosing four different sources of commercially available exogenous phytase increases the apparent ileal digestibility (AID) of CP and AA in a corn-soybean meal (SBM) based diet fed to growing pigs.

Authors: 
Publication Type: 

Standardized ileal digestibility of amino acids in canola meal fed to gestating and lactating sows

Velayudhan, Deepak Ettungapladi , Manik M. Hossain, Hans H. Stein, and C. Martin Nyachoti. 2019. Standardized ileal digestibility of amino acids in canola meal fed to gestating and lactating sows. Journal of Animal Science, 2019, 4219–4226. Link to full text.

Swine Nutrition

Stein H. H. 2019. Swine Nutrition. Feedstuffs reference issue & nutrition guide. September 2019, p 35 - 40. Link to full text.

Authors: 

Can the digestible indispensable amino acid score methodology decrease protein malnutrition

Bailey, H. M., H. H. Stein. 2019. Can the digestible indispensable amino acid score methodology decrease protein malnutrition. Animal Frontiers, Volume 9, Issue 4, October 2019, Pages 18–23. Link to full text.

Authors: 

Effects of heat treatment on digestibility of amino acids and concentration of metabolizable energy in soybean meal fed to pigs

Lee, S. A., M. S. F. Oliveira, W. B. Kwon, and H. H. Stein. 2019. Effects of heat treatment on digestibility of amino acids and concentration of metabolizable energy in soybean meal fed to pigs. Book of Abstracts. In: 1st International Feed Technology Congress, Cologne, Germany. p. 33. (Abstr.). Link to Abstract.

Authors: 
Publication Type: 

Pages