Digestibility

Effects of Increasing Dose of a Novel E. coli Phytase on Total Tract Digestibility of Minerals and Energy in Pigs

Lee, Su A., and H. H. Stein. 2022. Effects of Increasing Dose of a Novel E. coli Phytase on Total Tract Digestibility of Minerals and Energy in Pigs. J. Anim. Sci. 100(Suppl. 2): 36-37. doi.org/10.1093/jas/skac064.059. Link to Abstr.

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Effect of Increasing Levels of a Novel Consensus Bacterial 6-Phytase Variant on Ileal and Total Tract Digestibility of Nutrients in Diets Fed to Young Pigs.

Espinosa, C. D., D. Velayudhan, Y. Dersjant-Li, and H. H. Stein. 2022. Effect of Increasing Levels of a Novel Consensus Bacterial 6-Phytase Variant on Ileal and Total Tract Digestibility of Nutrients in Diets Fed to Young Pigs. J. Anim. Sci. 100(Suppl.2): 34–35. doi.org/10.1093/jas/skac064.056. Link to Abstr.

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Effects of a probiotic Bacillus strain on ileal digestibility of crude protein, dry matter, starch, energy and fat and total tract digestibility of energy and dietary fiber in diets fed to weanling pigs

Addition of probiotics to swine diets may improve gut health by modifying the microflora, which may help control pathogens, enhance immune response, and increase nutrient digestibility. Once consumed by the pig, probiotics enter the stomach where they are subjected to a low pH and pepsin. The Bacillus strain are metabolically inactive spores that are thermostable and survive at a low pH and, therefore, are thought to survive feed processing and digestion in the stomach. Addition of a Bacillus strain may enhance fermentation of dietary fiber in swine diets and, subsequently, increase the available energy from the diet in the form of volatile fatty acids. Bacillus strain also may degrade non-starch polysaccharides to reducing sugars that may serve as an energy source for the pig. A novel probiotic Bacillus toyonensis M15750 has been developed, but there are limited data to demonstrate the efficacy of this probiotic to increase nutrient digestibility. Therefore, an experiment was conducted to test the hypothesis that probiotic Bacillus toyonensis M15750 improve the apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of energy and nutrients when included in diets fed by weanling pigs.

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Ileal and total tract digestibility of energy and nutrients in pig diets supplemented with a novel consensus bacterial 6-phytase

The effect of microbial phytase on Ca and P digestibility in diets for pigs and poultry is well established. In poultry, it also appears that the effect of phytase in increasing amino acid (AA) digestibility is consistent, but that is not the case when phytase is added to diets for pigs. However, in many experiments, relatively low levels of phytase was used and it is not known if greater concentrations of phytase will result in a different result. A novel phytase has been recently developed; however, it is also not known if this phytase source can increase digestibility of AA and other nutrients. Therefore, an experiment was conducted to test the hypothesis that inclusion of increasing levels of the novel phytase in diets for growing pigs increases the apparent ileal digestibility (AID) of crude protein (CP) and AA, and the apparent total tract digestibility (ATTD) of gross energy (GE) and minerals.

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Effects of dietary levels of calcium, phosphorus, and 1-alphahydroxycholecalciferol on digestibility, retention of calcium and phosphorus, and concentration of metabolizable energy in diets fed to sows in late-gestation

Lee, Su A., and Hans H. Stein. 2022. Effects of dietary levels of calcium, phosphorus, and 1-alphahydroxycholecalciferol on digestibility, retention of calcium and phosphorus, and concentration of  metabolizable energy in diets fed to sows in late-gestation. Can. J. Anim. Sci. 102: 184–188 (2022) dx.doi.org/10.1139/cjas-2021-0018. Link to full text.

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Shiitake mycelium fermentation improves digestibility, nutritional value, flavor and functionality of plant proteins

Clark, Anthony J., Bhupendra K. Soni, Brendan Sharkey, Terry Acree, Edward Lavin, Hannah M. Bailey, Hans H. Stein, Ashley Han, Marc Elie, Marina Nadal. 2022. Shiitake mycelium fermentation improves digestibility, nutritional value, flavor and functionality of plant proteins. LWT - Food Science and Technology 156 (2022) 113065. doi.org/10.1016/j.lwt.2021.113065.

Comparative digestibility of energy, dry matter, and nutrients by gestating and lactating sows fed corn-soybean meal diets without or with full fat or defatted rice bran

The physiological stage of pigs may influence total tract digestibility of nutrients because the digestibility of energy and some nutrients increases as body weight increases, but the impact of physiological stage may be greater for high-fiber diets than for diets with less concentration of fiber. Full fat rice bran (FFRB) and defatted rice bran (DFRB) are produced in the rice milling process and is available for animal feeding. However, because of the high concentration of dietary fiber, FFRB and DFRB may be better suited for diets fed to sows than for diets for weanling or growing pigs, but there is a lack of data on the digestibility of energy and nutrients in FFRB and DFRB fed to sows. Therefore, the objective of this experiment was to test the null-hypothesis that the apparent total tract digestibility (ATTD) of gross energy (GE), dry matter (DM), organic matter (OM), neutral detergent fiber (NDF), and P in a corn-soybean meal diet and in diets containing FFRB or DFRB is not different between lactating sows and gestating sows if both groups are allowed to consume their diet on an ad libitum basis.

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Long-term steam conditioning is needed to maximize the nutritional value of expander-processed soybean expellers

Espinosa, Charmaine D., Maryane S. F. Oliveira, Joseph R. Limbach, Natalia S. Fanelli, Markus K. Wiltafsky-Martin, and Hans H. Stein. 2021. Long-term steam conditioning is needed to maximize the nutritional value of expander-processed soybean expellers. Can. J. Anim. Sci. 101: 704–714 (2021) dx.doi.org/10.1139/cjas-2021-0029. Link to full text.

Effect of phytase on mineral digestibility and growth performance of pigs fed diets with pharmacological levels of Zn

Espinosa, C. D., D. Velayudhan, Y. Dersjant-Li, J. Remus, H. H. Stein. 2021. Effect of phytase on mineral digestibility and growth performance of pigs fed diets with pharmacological levels of Zn. J. Anim. Sci. 99(Suppl. S3): 400-401. doi.org/10.1093/jas/skab235.727. Link to abstract.

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A Salute to PVT TIM HiLL: Indispensable Amino Acids and Global Human Health

Berg, Eric P., and Hans H. Stein. 2021. A Salute to PVT TIM HiLL: Indispensable Amino Acids and Global Human Health. Meat and Muscle Biology 2021.5(3): 4, 1–10. doi:10.22175/mmb.12925. Link to full text.

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The amino acid digestibility and digestible indispensable amino acid score for rapeseed protein isolate increases after moderate heating resulting in a protein quality similar to whey protein isolate

Rapeseed is the second most produced oilseed in the world after soybean, and after the oil is extracted, a protein-rich meal is the resulting byproduct containing greater concentrations of sulfur amino acids (AA; i.e., Met and Cys) and Lys compared with legumes and cereal grains. Rapeseed proteins have great potential as a high-quality plant-based protein for humans due to their well-balanced AA profile, high metabolic utilization of protein, a protein digestibility corrected amino acid score (PDCAAS) similar to soy and milk proteins, and easily separable antinutritional factors. However, to our knowledge, digestible indispensable amino acid score (DIAAS) has not been determined for rapeseed protein isolate and the level of processing required to concentrate rapeseed into a protein isolate warrants further evaluation of its protein quality. Therefore, this experiment was conducted to test the hypothesis that heat treatment of rapeseed protein isolate will increase the digestibility of AA by growing pigs and result in a DIAAS that is comparable to soy and animal protein isolates.

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Effects of increasing phytase dose on total tract digestibility of minerals and energy in pigs

In most plant feed ingredients, the majority of P is bound to phytate, which reduces digestibility of P in pigs, and therefore, the concentration of digestible P in these ingredients is relatively low. Phytate is negatively charged in the intestinal tract and can bind both endogenous and dietary nutrients, which results in precipitation of non-digestible nutrient-phytate complexes. Therefore, it is possible that the use of exogenous phytase can also increase the digestibility of other nutrients than P. As an example, addition of exogenous phytase to diets also releases Ca from phytate, and thus, increases the digestibility of Ca. However, it has not been conclusively demonstrated that phytase also increases the digestibility of energy-generating nutrients and other minerals in diets fed to pigs and inconsistent results among experiments have been reported. Therefore, the objective of this experiment was to test the hypothesis that increasing phytase dose increases the apparent total tract digestibility (ATTD) of minerals and gross energy (GE) in corn and soybean meal-based diets fed to growing pigs.

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Comparative digestibility and retention of calcium and phosphorus in normal- and high-phytate diets fed to gestating sows and growing pigs

Lee, S. A., M. R. Bedford, H. H. Stein. 2021. Comparative digestibility and retention of calcium and phosphorus in normal- and high-phytate diets fed to gestating sows and growing pigs. Animal Feed Science and Technology 280 (2021) 115084. doi.org/10.1016/j.anifeedsci.2021.115084. Link to full text.

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Ca and P digestibility values aren’t accurate for formulating sow diets

Lee, Su A and H. H. Stein. 2021. Ca and P digestibility values aren’t accurate for formulating sow diets. Pork Magazine, On-line edition, Sep. 9, 2021. Link to full text.

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Addition of hydrochloric acid to collection bags or collection containers did not change basal endogenous losses or ileal digestibility of amino acid in corn, soybean meal, or wheat middlings fed to growing pigs

Lee, Su A, Laia Blavi, Diego M. D. L. Navarro, and Hans H. Stein. 2021. Addition of hydrochloric acid to collection bags or collection containers did not change basal endogenous losses or ileal digestibility of amino acid in corn, soybean meal, or wheat middlings fed to growing pigs. Animal Bioscience Vol. 34, No. 10:1632-1642 October 2021. doi.org/10.5713/ab.20.0838.

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Digestibility of amino acids and concentration of metabolizable energy are greater in high-oil corn than in conventional corn when fed to growing pigs

Espinosa, C. D., N. S. Fanelli, H. H. Stein. 2021. Digestibility of amino acids and concentration of metabolizable energy are greater in high-oil corn than in conventional corn when fed to growing pigs. Animal Feed Science and Technology 280: 115040. doi.org/10.1016/j.anifeedsci.2021.115040. Link to full text.

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Influence of a novel consensus bacterial 6-phytase variant on mineral digestibility and bone ash in young growing pigs fed diets with different concentrations of phytate-bound phosphorus

Espinosa, Charmaine D., Maryane S. F. Oliveira, Deepak E. Velayudhan, Yueming Dersjant-Li, Hans H. Stein. 2021. Influence of a novel consensus bacterial 6-phytase variant on mineral digestibility and bone ash in young growing pigs fed diets with different concentrations of phytate-bound phosphorus. Journal of Animal Science, 2021, Vol. 99, No. 8, 1–12. doi.org/10.1093/jas/skab211.

Soybean meal sourced from Argentina, Brazil, China, India and USA as an ingredient in practical diets for Pacific white shrimp Litopenaeus vannamei

Galkanda-Arachchige, Harsha S. C., Hans H. Stein, and D. Allen Davis. 2021. Soybean meal sourced from Argentina, Brazil, China, India and USA as an ingredient in practical diets for Pacific white shrimp Litopenaeus vannamei. Aquaculture Nutrition. 2021;27:1103–1113. doi: 10.1111/anu.13251. Link to full text.

Effect of phytase on weaning piglet performance when fed diets supplemented with pharmacological levels of Zn

Pharmacological levels of Zn (i.e., 2,000 to 3,000 mg/kg) is often included in diets for weanling pigs to prevent post-weaning diarrhea. However, pharmacological levels of Zn may reduce microbial phytase efficacy by chelating the phytate molecule, which subsequently prevents access for phytase.  However, it is possible that this effect can be reduced by adding more phytase to diets. Therefore, an experiment was conducted to test the hypothesis that inclusion of increasing levels of phytase increases pig growth performance and mineral digestibility in diets with 3,000 mg/kg of Zn.

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Concentrations of digestible amino acids in co-products from threonine and tryptophan fermentation are greater than in soybean meal

Espinosa, Charmaine D., M. S. F. Oliveira, J. K. Htoo, H. H. Stein. 2021. Concentrations of digestible amino acids in co-products from threonine and tryptophan fermentation are greater than in soybean meal. Animal Feed Science and Technology 277 (2021) 114948. doi.org/10.1016/j.anifeedsci.2021.114948. Link to full text.

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