Lagos, L. V., J. C. Woodworth, S. W. Kim, and H. H. Stein. 2023. Short communication: Commercial diets for pigs in the United States contain more calcium than formulated. J. Anim. Sci.101:1-5. doi.org/10.1093/jas/skad102. Link to full text.

Lagos, V., M. Bedford, and H. H. Stein. 2022. The digestibility of energy and nutrients and the efficiency of phytase to degrade phytate is influenced by pig body weight. 15th International Symposium on Digestive Physiology of Pigs. Animal - Science Proceedings 13(Issue 2): 174. Link to abstract.

Lagos, L. Vanessa, Mike R. Bedford, and Hans H. Stein. 2021. Increased microbial phytase increased phytate destruction, plasma inositol, and feed efficiency of weanling pigs, but reduced dietary calcium and phosphorus did not affect gastric pH or fecal score and reduced growth performance and bone ash. J. Anim. Sci. 99: 12, 1-13. doi.org/10.1093/jas/skab333.

The limited capacity for weanling pigs to secret HCl in the stomach may be exacerbated by inclusion of ingredients with high acid binding capacity such as limestone and monocalcium phosphate. As a consequence, reducing the amount of these 2 ingredients in diets for weanling pigs may contribute to a stable low pH for proper pepsin activity and increased action of microbial phytase. Inclusion of high doses of phytase that results in increased phytate degradation and increased release of Ca, P, and inositol may also be beneficial to newly weaned pigs. Therefore, an experiment was conducted to test the hypothesis that lowering dietary Ca and P reduces gastric pH and diarrhea of weanling pigs, but microbial phytase may overcome negative effects of low Ca and P on growth performance and bone ash.

Lagos, L. Vanessa, Su A Lee, Mike R. Bedford, and Hans H. Stein. 2021. Formulation of diets for pigs based on a ratio between digestible calcium and digestible phosphorus results in reduced excretion of calcium in urine without affecting retention of calcium and phosphorus compared with formulation based on values for total calcium. Journal of Animal Science, 2021, Vol. 99, No. 5, 1–7. doi:10.1093/jas/skab138.

Lagos, L. Vanessa, Su A Lee, Mike R. Bedford, and Hans H. Stein. 2021. Formulating diets based on digestible calcium instead of total calcium does not affect growth performance or carcass characteristics, but microbial phytase ameliorates bone resorption caused by low calcium in diets fed to pigs from 11 to 130 kg. Journal of Animal Science, 2021, Vol. 99, No. 3, 1–11. doi:10.1093/jas/skab057.

To obtain accurate values for metabolizable energy (ME) in diets and ingredients, an accurate estimation of gross energy (GE) in urine is required. Urine samples are often prepared following the freeze-dried cotton-plastic bag method and GE is determined by the ignition of the sample in a bomb calorimeter. However, the concentration of GE in urine can also be determined by dripping urine on a cellulose pellet, which can then be ignited in a bomb calorimeter. Alternatively, energy in urine can be calculated from the concentration of N in urine assuming that all energy in urine originates from N. The latter 2 methods may be less expensive and less time consuming than using the freeze-dried cotton-plastic bag method. Likewise, oven drying instead of freeze drying of samples may reduce time and cost of the drying procedure. However, to our knowledge, no data comparing values for GE in urine and ME in diets among different sample preparation and drying methods are available. Therefore, the objective of this study was to test the hypothesis that there are no differences in GE of urine or ME values of diets among sample preparation methods (freeze-dried cotton-plastic bag, undried cellulose pellet, or N value method). The second hypothesis was that drying method (freeze drying or oven drying) of cotton-plastic bag urine samples from pigs do not influence analyzed the GE in urine or calculated ME in diets.

Lancheros, Paola, Vanessa Lagos, Hans H Stein. 2020. Enhanced torula yeast has increased nutritional value compared to fish meal when fed to weanling pigs. J. Anim. Sci.  98(Suppl. 3): 62. doi.org/10.1093/jas/skaa054.112. (Abstr.). Link to abstract.

Espinosa, Charmaine D., L. Vanessa Lagos, and Hans H. Stein. 2020. Effect of torula yeast on growth performance, diarrhea incidence, and blood characteristics in weanling pigs. Journal of Animal Science, 2020, Vol. 98, No. 10, 1–9. doi:10.1093/jas/skaa307. Link to full text.