Digestible indispensable amino acid score

Values for digestible indispensable amino acid score (DIAAS) determined in pigs for breakfast cereals and milk are additive in combined breakfast cereal-milk meals

Breakfast cereals are usually consumed with milk as a breakfast meal. To meet requirements for amino acids (AA), higher quality proteins are needed to complement the protein in cereals to provide a meal that is adequate in all indispensable AA. The digestible indispensable amino acid score (DIAAS) method used to determine protein quality allows for calculation of the protein value of individual ingredients and mixed meals consisting of several proteins. Values for DIAAS are based on values for apparent ileal digestibility (AID) of AA that are corrected for the basal endogenous loss of each individual AA, resulting in values defined as standardized ileal digestibility (SID). Values for SID of AA are additive in mixed meals because these values are independent of basal endogenous losses. Consequently, it is expected that DIAAS obtained for individual food ingredients are additive in a mixed meal, but data to demonstrate this have not been reported. Therefore, the objective of this experiment was to test the hypothesis that AA in milk complement AA in breakfast cereals to provide a balanced meal and that DIAAS in individual foods are additive in a combined meal.  

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Determination of true ileal amino acid digestibility in the growing pig for calculation of digestible indispensable amino acid score (DIAAS)

Hodgkinson, Suzanne M., Hans H. Stein, Sonja de Vries, Wouter H. Hendriks, and Paul J. Moughan. 2020. Determination of true ileal amino acid digestibility in the growing pig for calculation of digestible indispensable amino acid score (DIAAS). The Journal of Nutrition. First published online, 2020. doi.org/10.1093/jn/nxaa210.

Pork Products Have Digestible Indispensable Amino Acid Scores (DIAAS) That Are Greater Than 100 When Determined in Pigs, but Processing Does Not Always Increase DIAAS

Bailey, Hannah M., John K. Mathai, Eric P. Berg, and Hans H. Stein. 2020. Pork Products Have Digestible Indispensable Amino Acid Scores (DIAAS) That Are Greater Than 100 When Determined in Pigs, but Processing Does Not Always Increase DIAAS. The Journal of Nutrition. Nutrient Physiology, Metabolism, and Nutrient-Nutrient Interactions. doi: https://doi.org/10.1093/jn/nxz284.

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Digestible indispensable amino acid score (DIAAS) and protein digestibility corrected amino acid score (PDCAAS) in oat protein concentrate measured in 20- to 30-kilogram pigs

Abelilla, J. J., Liu, Y., and H. H. Stein. 2018. Digestible indispensable amino acid score (DIAAS) and protein digestibility corrected amino acid score (PDCAAS) in oat protein concentrate measured in 20- to 30-kilogram pigs. J. Sci. Food Agric. 98:410–414. Link to abstract

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Values for digestible indispensable amino acid scores (DIAAS) for some dairy and plant proteins may better describe protein quality than values calculated using the concept for protein digestibility-corrected amino acid scores (PDCAAS)

Mathai, J. K., Y. Liu, and H. H. Stein. 2017. Values for digestible indispensable amino acid scores (DIAAS) for some dairy and plant proteins may better describe protein quality than values calculated using the concept for protein digestibility-corrected amino acid scores (PDCAAS). Brit. J. Nutr. 117:490-499. Link to full text (.pdf)

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Use of the pig to determine digestible indispensable amino acid scores (DIAAS) in human foods

Stein, H. H., and J. K. Mathai. 2016. Use of the pig to determine digestible indispensable amino acid scores (DIAAS) in human foods. Proc. 5th Intl. Symp. Energy and Protein. Krakow, Poland, Sep. 12 to 15, 2016.

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Evaluating protein quality of human foods using the pig as a model

Stein, H. H. 2016. Evaluating protein quality of human foods using the pig as a model. J. Anim. Sci. 94(Suppl. 2):20 (Abstr.) Link to abstract (.pdf)

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Digestible indispensable amino acid score and digestible amino acids in eight cereal grains

Cervantes-Pahm, S. K., Y. Liu, and H. H. Stein. 2014. Digestible indispensable amino acid score and digestible amino acids in eight cereal grains. Brit. J. Nutr. 111:1663-1672. Link to full text (.pdf)