Phytase

Effects of a novel bacterial phytase expressed in Aspergillus oryzae on digestibility of calcium and phosphorus in diets fed to weanling or growing pigs

Almeida, F. N., R. C. Sulabo, and H. H. Stein. 2013. Effects of a novel bacterial phytase expressed in Aspergillus oryzae on digestibility of calcium and phosphorus in diets fed to weanling or growing pigs. J. Anim. Sci. Biotechnol. 4:8. Link to full text (.pdf)

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Determination of endogenous intestinal losses of calcium and apparent and true total tract digestibility of calcium in canola meal fed to growing pigs

When formulating diets for pigs, it is more accurate to use values for standardized or true nutrient digestibility than values for apparent nutrient digestibility because the former are additive in mixed diets. No values for standardized or true total tract digestibility of calcium in pigs have been reported. The true total tract digestibility (TTTD) of a nutrient is calculated by correcting apparently total tract digestibility (ATTD) by total endogenous losses, which may be estimated using a regression procedure. To our knowledge, no measurements of the endogenous loss of calcium in pigs have been reported. An experiment was, therefore,  performed to measure endogenous loss of calcium and to determine TTTD of calcium in growing pigs, and to investigate if  the addition of microbial phytase to the diets affects TTTD of calcium. In addition, calcium retention was measured in pigs fed diets containing varying levels of calcium with or without microbial phytase.

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Digestibility of phosphorus by growing pigs of fermented and conventional soybean meal without and with microbial phytase

Rojas, O. J. and H. H. Stein. 2012. Digestibility of phosphorus by growing pigs of fermented and conventional soybean meal without and with microbial phytase. J. Anim. Sci. 90:1506-1512. Link to full text (.pdf)

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Effects of graded levels of microbial phytase on the standardized total tract digestibility of phosphorus in corn and corn coproducts fed to pigs

Almeida, F. N. and H. H. Stein. 2012. Effects of graded levels of microbial phytase on the standardized total tract digestibility of phosphorus in corn and corn coproducts fed to pigs. J. Anim Sci. 90:1262-1269. Link to full text (.pdf)

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Correct use of microbial phytase in diets fed to pigs

By Dr. Hans H. Stein

November, 2011

Microbial phytase is often used in diets fed to pigs because it is well established that the digestibility of phosphorus in many ingredients is increased if microbial phytase is used in the diet. The reason for this increase in digestibility is that microbial phytase has the ability to release some of the phosphorus that is bound in the phytate complex in many feed ingredients. Without phytase in the diet, most of the phytate-bound phosphorus is not digested by the pigs and is instead excreted in the manure.

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Effects of phytase on standardized total tract digestibility of P in copra expellers, palm kernel expellers, and palm kernel meal fed to growing pigs

Almaguer, B. L., R. C. Sulabo, and H. H. Stein. 2011. Effects of phytase on standardized total tract digestibility of P in copra expellers, palm kernel expellers, and palm kernel meal fed to growing pigs. J. Anim. Sci. 89(E-Suppl. 1):187 (Abstr.) Link to abstract (.pdf)

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Effects of graded levels of microbial phytase on the standardized total tract digestibility of phosphorus in corn and corn co-products

Corn contains approximately 0.26% phosphorus, but most of this phosphorus is bound to phytic acid, or phytate, and thus is not bioavailable to pigs because they lack the enzyme phytase. Corn-based diets can be supplemented with inorganic phosphorus to help meet pigs' phosphorus needs; however, rising costs of inorganic phosphorus makes this approach increasingly uneconomical. Helping pigs digest more of the phosphorus that naturally occurs in the diet could provide a cost-effective alternative to inorganic phosphorus supplementation while also helping to reduce the environmental burden of excreted phosphorus. An experiment was conducted to determine how adding graded levels of microbial phytase affected the standardized total tract digestibility (STTD) of phosphorus in corn and corn co-products. From these data, regression equations were developed to predict the response to adding different levels of phytase to each ingredient.

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Phosphorus digestibility and energy concentration of enzyme-treated and conventional soybean meal fed to weanling pigs

Goebel, K. P., and H. H. Stein. 2011. Phosphorus digestibility and energy concentration of enzyme-treated and conventional soybean meal fed to weanling pigs. J. Anim. Sci. 89:764–772. Link to full text (.pdf)

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Effects of a novel phytase on phosphorus digestibility in corn-soybean meal diets fed to weanling and growing pigs

Almeida, F. N. and H. H. Stein. 2010. Effects of a novel phytase on phosphorus digestibility in corn-soybean meal diets fed to weanling and growing pigs. J. Anim. Sci. 88(E-Suppl. 2):861 (Abstr.) Link to abstract (.pdf)

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Effects of graded levels of phytase on the apparent and standardized total tract digestibility of phosphorus in corn and corn co-products

Almeida, F. N. and H. H. Stein. 2010. Effects of graded levels of phytase on the apparent and standardized total tract digestibility of phosphorus in corn and corn co-products. J. Anim. Sci. 88(E-Suppl. 2):378 (Abstr.) Link to abstract (.pdf)

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Performance and phosphorus balance of pigs fed diets formulated on the basis of values for standardized total tract digestibility of phosphorus

Almeida, F. N. and H. H. Stein. 2010. Performance and phosphorus balance of pigs fed diets formulated on the basis of values for standardized total tract digestibility of phosphorus. J. Anim Sci. 88:2968-2977. Link to full text (.pdf)

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Finding phosphorus solutions for weaned-pig diets

Almeida, F. N. and H. H. Stein. 2010. Finding phosphorus solutions for weaned-pig diets. Pages 24-25 in Pork Magazine, August 2010. Link to full text (.pdf)

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Distillers dried grains with solubles (DDGS) and the use of phytase to address digestibility issues in pigs

Stein, H. H. 2010. Distillers dried grains with solubles (DDGS) and the use of phytase to address digestibility issues in pigs. In Proc. 46th Eastern Nutr. Conf., May 12-13, 2010, Guelph, Ontario. Link to full text (.pdf)

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Effects of dietary concentration of P and microbial phytase on the digestibility of amino acids and energy by growing pigs

Pahm, A. A., G. I. Petersen, N. R. Augspurger, and H. H. Stein. 2009. Effects of dietary concentration of P and microbial phytase on the digestibility of amino acids and energy by growing pigs. J. Anim. Sci. 87 (E-Suppl. 3):92 (Abstr.) Link to abstract (.pdf)

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Effects of a Pichia-expressed phytase on performance and P excretion of growing pigs

McGinnis, L. M., M. R. Widmer, C. L. Wright, T. M. Parr, and H. H. Stein. 2007. Effects of a Pichia-expressed phytase on performance and P excretion of growing pigs. J. Anim. Sci. 85(Suppl. 1):438 (Abstr.) Link to abstract (.pdf)

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Effects of feeding an evolved E.coli-derived phytase to weanling and growing pigs

McGinnis, L. M., M. R. Widmer, C. L. Wright, T. M. Parr, and H. H. Stein. 2007. Effects of feeding an evolved E.coli-derived phytase to weanling and growing pigs. J. Anim. Sci. 85(Suppl. 2):112 (Abstr.) Link to abstract (.pdf)

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Site of activity within the digestive tract of growing pigs of an evolved E.coli-derived and a fungal-derived commercial phytase

McGinnis, L. M., M. R. Widmer, C. L. Wright, T. M. Parr, and H. H. Stein. 2007. Site of activity within the digestive tract of growing pigs of an evolved E.coli-derived and a fungal-derived commercial phytase. J. Anim. Sci. 85(Suppl. 2):87 (Abstr.)  Link to abstract (.pdf)

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Digestibility of phosphorus in field peas by growing pigs

Geraets, L. L., M. G. Boersma, and H. H. Stein. 2006. Digestibility of phosphorus in field peas by growing pigs. J. Anim. Sci. 84 (Suppl. 2):116 (Abstr.) Link to abstract (.pdf)

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Site of digestibility of protein and phosphorus by growing pigs fed diets without or with microbial phytase

Geraets, L. L., M. G. Boersma, and H. H. Stein. 2005. Site of digestibility of protein and phosphorus by growing pigs fed diets without or with microbial phytase. J. Anim. Sci. 83(Suppl. 1): 389 (Abstr.) Link to abstract (.pdf)

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Effect of body weight and reproductive status on phosphorus digestibility and phytase efficacy in pigs

Sulabo, R., R. C. Thaler, and H. H. Stein. 2004. Effect of body weight and reproductive status on phosphorus digestibility and phytase efficacy in pigs. J. Anim. Sci. 82(Suppl. 2):82 (Abstr.) Link to abstract (.pdf)

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