Phytase

Effects of graded levels of phytase on digestibility of nutrients, growth performance, and bone ash in corn and soybean meal based diets fed to pigs

Lee, S. A., and H. H. Stein. 2019. Effects of graded levels of phytase on digestibility of nutrients, growth performance, and bone ash in corn and soybean meal based diets fed to pigs. In: 80th Minnesota Nutrition Conference, Mankato, MN, Sep. 18-19, 2019. P. 25. (Abstr.). Link to abstract

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PSIV-13 Basal endogenous loss, standardized total tract digestibility, and retention of Ca in sows change throughout gestation, but microbial phytase reduces basal endogenous loss of Ca by gestating sows

Lee Su A., Carrie L. Walk, Hans H. Stein. 2019. PSIV-13 Basal endogenous loss, standardized total tract digestibility, and retention of Ca in sows change throughout gestation, but microbial phytase reduces basal endogenous loss of Ca by gestating sows. Journal of Animal Science, Volume 97, Issue Supplement_2, July 2019, Pages 185–186. (Abstr.). Link to abstract.

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PSIII-18 Standardized total tract digestibility of Ca by growing pigs in different sources of calcium carbonate and dicalcium phosphate

Lee Su A., Carrie L. Walk, Hans H. Stein. 2019. PSIII-18 Standardized total tract digestibility of Ca by growing pigs in different sources of calcium carbonate and dicalcium phosphate. Journal of Animal Science, Volume 97, Issue Supplement_2, July 2019, Pages 173–174. (Abstr.). Link to abstract.

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Effects of a novel corn-expressed E. coli phytase on digestibility of calcium and phosphorous, growth performance, and bone ash in young growing pigs

Blavi, Laia, Cristhiam J. Muñoz, Jonathan N. Broomhead, and Hans H. Stein. 2019. Effects of a novel corn-expressed E. coli phytase on digestibility of calcium and phosphorous, growth performance, and bone ash in young growing pigs. J. Anim. Sci. 2019.97:3390–3398. Link to full text.

Standardized total tract digestibility of calcium varies among sources of calcium carbonate, but not among sources of dicalcium phosphate, but microbial phytase increases calcium digestibility in calcium carbonate

Lee, Su A, L. Vanessa Lagos, Carrie L. Walk, and Hans H. Stein. 2019. Standardized total tract digestibility of calcium varies among sources of calcium carbonate, but not among sources of dicalcium phosphate, but microbial phytase increases calcium digestibility in calcium carbonate. J. Anim. Sci. 2019.97:3440–3450.

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Phosphorus and energy digestibility of Fermex 200 (fermented soybean meal) fed to weanling pigs

Soybean meal (SBM) is one of the most important protein sources in swine diets. However, most P in SBM is bound to phytate, which increases inclusion of inorganic P in diets for pigs. Use of microbial phytase may hydrolyze phytate and subsequently improve P absorption. Fermex 200 (Purina Animal Nutrition, Shoreview, MN, USA) is a new source of fermented SBM that may serve as an alternative to other protein sources in diets fed to pigs. However, there are at this point no data for effects of adding phytase to diets containing Fermex 200 and no data for digestible energy (DE) and metabolizable energy (ME) concentrations of Fermex 200.

Therefore, 2 experiments were conducted to test the hypothesis that inclusion of 1,000 phytase units (FTU)/kg of microbial phytase improves the standardized total tract digestibility (STTD) of P in conventional SBM and Fermex 200. The second hypothesis was that the STTD of P, as well as concentrations of DE and ME in Fermex 200 are greater than in conventional SBM.

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Zinc oxide and microbial phytase may reduce calcium and phosphorus digestibility

Blavi, L., and H. H. Stein. 2017. Zinc oxide and microbial phytase may reduce calcium and phosphorus digestibility. National Hog Farmer, Online edition, March 30, 2017. Link to full text.

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Basal endogenous loss, standardized total tract digestibility of calcium in calcium carbonate, and retention of calcium in gestating sows change during gestation, but microbial phytase reduces basal endogenous loss of calcium

Lee, Su A, L. Vanessa Lagos, Carrie L. Walk, and Hans H. Stein. 2019. Basal endogenous loss, standardized total tract digestibility of calcium in calcium carbonate, and retention of calcium in gestating sows change during gestation, but microbial phytase reduces basal endogenous loss of calcium. J. Anim. Sci. 2019.97:1712–1721. Link to full text.

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Effects of microbial phytase on mucin synthesis, gastric protein hydrolysis, and degradation of phytate along the gastrointestinal tract of growing pigs

Mesina Von G. R., L. Vanessa Lagos, Rommel C. Sulabo, Carrie L. Walk, and Hans H. Stein. 2019. Effects of microbial phytase on mucin synthesis, gastric protein hydrolysis, and degradation of phytate along the gastrointestinal tract of growing pigs. J. Anim. Sci. 2019.97:756–767. Link to full text.

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Increasing levels of microbial phytase increases the digestibility of energy and minerals in diets fed to pigs

Arredondo Mónica A., Gloria A. Casas, Hans H. Stein. 2019. Increasing levels of microbial phytase increases the digestibility of energy and minerals in diets fed to pigs. Anim. Feed Sci. Technol. 248: 27 - 36. Link to full text.

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Effects of increasing concentrations of an Escherichia coli phytase on the apparent ileal digestibility of amino acids and the apparent total tract digestibility of energy and nutrients in corn-soybean meal diets fed to growing pigs

Yue She, J. Chris Sparks, and Hans H. Stein. 2018. Effects of increasing concentrations of an Escherichia coli phytase on the apparent ileal digestibility of amino acids and the apparent total tract digestibility of energy and nutrients in corn-soybean meal diets fed to growing pigs. J. Anim. Sci. 96:2804–2816.

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The level of feed intake does not influence digestibility of calcium and phosphorus in diets fed to gestating sows, but gestating sows have reduced digestibility of calcium and phosphorus compared with growing gilts

Lee, S. A., G. A. Casas, and H. H. Stein. 2018. The level of feed intake does not influence digestibility of calcium and phosphorus in diets fed to gestating sows, but gestating sows have reduced digestibility of calcium and phosphorus compared with growing gilts. Can. J. Anim. Sci. 98:591-594. Link to abstract

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Effects of a novel phytase on growth performance, bone measurements, and Ca and P digestibility in diets fed to growing pigs

Blavi, L., J. N. Broomhead, and H. H. Stein. 2018. Effects of a novel phytase on growth performance, bone measurements, and Ca and P digestibility in diets fed to growing pigs. J. Anim. Sci. 96(Suppl. 2):163 (Abstr.) Link to abstract (.pdf)

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Comparison between a novel phytase and a commercial phytase on growth performance and bone measurements in diets fed to growing pigs

Munoz Alfonso, C. J., L. Blavi, J. N. Broomhead, and H. H. Stein. 2018. Comparison between a novel phytase and a commercial phytase on growth performance and bone measurements in diets fed to growing pigs. J. Anim. Sci. 96(Suppl. 2):147-148 (Abstr.) Link to abstract (.pdf)

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Effects of GraINzyme and AxtraPhy phytases in restoring performance and bone ash in pigs fed low-phosphorus and calcium diets

Last month's newsletter included a research report on research into a novel E. coli phytase expressed in corn, called GraINzyme. Results of that research indicated that adding GraINzyme phytase to diets fed to young growing pigs increased growth performance, digestibility of calcium and phosphorus, and bone mineralization. The comparative effects of GraINzyme and a commercial phytase was not tested in the previous study. Therefore, an experiment was conducted to compare effects of addition of GraINzyme phytase to the commercial phytase AxtraPhy in diets fed to growing pigs.

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Effect on growth performance and bone mineralization of GraINzyme phytase in diets fed to growing pigs

Most of the phosphorus in corn and other plant-based feed ingredients fed to pigs is bound to phytate. Pigs do not secrete phytase in adequate quantities to liberate significant amounts of phytate-bound phosphorus, meaning that most of the phosphorus in these ingredients is not biologically available. Calcium digestibility is also negatively affected by the presence of phytate in feed ingredients. One way for producers to ensure that pigs' requirements are met is to add supplementary phosphorus to diets. However, this adds to the cost of diets. The excretion of phytate-bound phosphorus also may contribute to algae overgrowth in waterways.

Another approach is to add supplemental phytase to the diets, to allow pigs to better utilize the phosphorus present in the ingredients. Phytases can be obtained from plants, animals, or microorganisms (bacteria, yeasts, and fungi).

GraINzyme is a source of phytase produced by genetically modified corn plants with genes derived from E. coli. In this experiment, the effect on growth performance, apparent total tract digestibility (ATTD) of calcium and phosphorus, and bone mineralization of adding GraINzyme to weanling pig diets was tested.

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Effects of graded levels of an Escherichia coli phytase on growth performance, apparent total tract digestibility of phosphorus, and on bone parameters of weanling pigs fed phosphorus-deficient corn-soybean meal based diets

She, Y., Y. Liu, J. C. González-Vega, and H. H. Stein. 2017. Effects of graded levels of an Escherichia coli phytase on growth performance, apparent total tract digestibility of phosphorus, and on bone parameters of weanling pigs fed phosphorus-deficient corn-soybean meal based diets. Anim. Feed Sci. Technol. 232:102-109. Link to abstract

Effects of exogenous phytase supplementation on phosphorus metabolism and digestibility of beef cattle

Long, C. J., L. B. Kondratovich, M. F. Westphalen, H. H. Stein, and T. L. Felix. 2017. Effects of exogenous phytase supplementation on phosphorus metabolism and digestibility of beef cattle. Transl. Anim. Sci. 1:168-178. Link to full text (.pdf)

Effects of graded levels of microbial phytase on apparent total tract digestibility of calcium and phosphorus and standardized total tract digestibility of phosphorus in four sources of canola meal and in soybean meal fed to growing pigs

She, Y., Y. Liu, and H. H. Stein. 2017. Effects of graded levels of microbial phytase on apparent total tract digestibility of calcium and phosphorus and standardized total tract digestibility of phosphorus in four sources of canola meal and in soybean meal fed to growing pigs. J. Anim. Sci. 95:2061-2070. Link to full text (.pdf)

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Effects of zinc oxide and microbial phytase on standardized total tract digestibility of calcium in maize-based diets fed to growing pigs

Blavi, L., D. Solà-Oriol, J. F. Pérez, and H. H. Stein. 2017. Effects of zinc oxide and microbial phytase on standardized total tract digestibility of calcium in maize-based diets fed to growing pigs. J. Anim. Sci. 95(Suppl. 5):85 (Abstr.) Link to abstract (.pdf)

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