Phytase

Effect of microbial phytase on digestibility of phosphorus in seven sources of sunflower meal fed to growing pigs

The majority of P in oilseed co-products is bound to phytate; however, pigs do not synthesize adequate amount of endogenous phytate to liberate the P bound to phytate and the digestibility of P in sunflower meal, therefore is low. Values for ATTD and STTD of P in sunflower meal (SFM) without and with phytase have been reported, but there are no comparative values for the ATTD and STTD of P in sunflower co-products produced in different parts of the world. Therefore, the objective of this experiment was to determine the ATTD and the STTD of P in different sources of sunflower co-products, and to test the hypothesis that regardless of source, microbial phytase increases the digestibility of P in sunflower co-products fed to young pigs.

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Phytate breakdown, nutrient digestibility, plasma metabolites, and bone ash of pigs fed increasing phytase levels for a long adaptation period

Lagos, V., M. Bedford, and H. H. Stein. 2022. Phytate breakdown, nutrient digestibility, plasma metabolites, and bone ash of pigs fed increasing phytase levels for a long adaptation period. 15th International Symposium on Digestive Physiology of Pigs. Animal - Science Proceedings 13(Issue 2): 174-175. Link to abstract.

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Effects of Phosphorus Level and Increasing Phytase Dose on Basal Endogenous Loss of Calcium and Balance of Phosphorus in Pigs Fed Diets Containing Phytate P at Commercial Levels

Nelson, M. E., Su A Lee, Y. Dersjant-Li, D. Velayudhan, J. C. Remus, H. H. Stein. 2022. Effects of Phosphorus Level and Increasing Phytase Dose on Basal Endogenous Loss of Calcium and Balance of Phosphorus in Pigs Fed Diets Containing Phytate P at Commercial Levels. J. Anim. Sci. 100(Suppl. 2): 165–166. doi.org/10.1093/jas/skac064.282.

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Effect of Increasing Levels of a Novel Consensus Bacterial 6-Phytase Variant on Ileal and Total Tract Digestibility of Nutrients in Diets Fed to Young Pigs.

Espinosa, C. D., D. Velayudhan, Y. Dersjant-Li, and H. H. Stein. 2022. Effect of Increasing Levels of a Novel Consensus Bacterial 6-Phytase Variant on Ileal and Total Tract Digestibility of Nutrients in Diets Fed to Young Pigs. J. Anim. Sci. 100(Suppl.2): 34–35. doi.org/10.1093/jas/skac064.056. Link to Abstr.

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Novel bacterial phytase impact on pigs

Espinosa, C. D., and H. H. Stein. 2022. Novel bacterial phytase impact on pigs. National Hog Farmer, On-line edition, March 31, 2022. Link to full text.

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Ileal and total tract digestibility of energy and nutrients in pig diets supplemented with a novel consensus bacterial 6-phytase

The effect of microbial phytase on Ca and P digestibility in diets for pigs and poultry is well established. In poultry, it also appears that the effect of phytase in increasing amino acid (AA) digestibility is consistent, but that is not the case when phytase is added to diets for pigs. However, in many experiments, relatively low levels of phytase was used and it is not known if greater concentrations of phytase will result in a different result. A novel phytase has been recently developed; however, it is also not known if this phytase source can increase digestibility of AA and other nutrients. Therefore, an experiment was conducted to test the hypothesis that inclusion of increasing levels of the novel phytase in diets for growing pigs increases the apparent ileal digestibility (AID) of crude protein (CP) and AA, and the apparent total tract digestibility (ATTD) of gross energy (GE) and minerals.

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Increased microbial phytase increased phytate destruction, plasma inositol, and feed efficiency of weanling pigs, but reduced dietary calcium and phosphorus did not affect gastric pH or fecal score and reduced growth performance and bone ash

Lagos, L. Vanessa, Mike R. Bedford, and Hans H. Stein. 2021. Increased microbial phytase increased phytate destruction, plasma inositol, and feed efficiency of weanling pigs, but reduced dietary calcium and phosphorus did not affect gastric pH or fecal score and reduced growth performance and bone ash. J. Anim. Sci. 99: 12, 1-13. doi.org/10.1093/jas/skab333.

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Effects of phosphorus level and increasing phytase dose on basal endogenous loss of calcium and balance of phosphorus in pigs fed diets containing phytate P at commercial level

In plant-based feed ingredients there is a considerable amount of P bound to phytate, limiting the amount of P that is available for utilization, but inclusion of microbial phytase in pig diets increases the digestibility of P. The negatively charged phytate molecule can chelate Ca cations resulting in formation of insoluble Ca-phytate complexes. Degradation of phytate by microbial phytase may prevent formation of these non-digestible complexes, resulting in increased Ca digestibility. It is also possible that use of exogenous phytase reduces endogenous loss of Ca. If indeed the reduced endogenous loss of Ca is a result of degradation of phytate, it is expected that increased doses of dietary phytase will linearly reduce endogenous losses of Ca, but this hypothesis has not been experimentally verified. Therefore, this experiment was conducted to test the hypothesis that increasing dietary phytase reduces basal endogenous loss of Ca and increases digestibility of P in growing pigs.

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Effect of phytase on mineral digestibility and growth performance of pigs fed diets with pharmacological levels of Zn

Espinosa, C. D., D. Velayudhan, Y. Dersjant-Li, J. Remus, H. H. Stein. 2021. Effect of phytase on mineral digestibility and growth performance of pigs fed diets with pharmacological levels of Zn. J. Anim. Sci. 99(Suppl. S3): 400-401. doi.org/10.1093/jas/skab235.727. Link to abstract.

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Effects of increasing phytase dose on total tract digestibility of minerals and energy in pigs

In most plant feed ingredients, the majority of P is bound to phytate, which reduces digestibility of P in pigs, and therefore, the concentration of digestible P in these ingredients is relatively low. Phytate is negatively charged in the intestinal tract and can bind both endogenous and dietary nutrients, which results in precipitation of non-digestible nutrient-phytate complexes. Therefore, it is possible that the use of exogenous phytase can also increase the digestibility of other nutrients than P. As an example, addition of exogenous phytase to diets also releases Ca from phytate, and thus, increases the digestibility of Ca. However, it has not been conclusively demonstrated that phytase also increases the digestibility of energy-generating nutrients and other minerals in diets fed to pigs and inconsistent results among experiments have been reported. Therefore, the objective of this experiment was to test the hypothesis that increasing phytase dose increases the apparent total tract digestibility (ATTD) of minerals and gross energy (GE) in corn and soybean meal-based diets fed to growing pigs.

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Influence of a novel consensus bacterial 6-phytase variant on mineral digestibility and bone ash in young growing pigs fed diets with different concentrations of phytate-bound phosphorus

Espinosa, Charmaine D., Maryane S. F. Oliveira, Deepak E. Velayudhan, Yueming Dersjant-Li, Hans H. Stein. 2021. Influence of a novel consensus bacterial 6-phytase variant on mineral digestibility and bone ash in young growing pigs fed diets with different concentrations of phytate-bound phosphorus. Journal of Animal Science, 2021, Vol. 99, No. 8, 1–12. doi.org/10.1093/jas/skab211.

Reduced concentrations of limestone and monocalcium phosphate in diets without or with microbial phytase did not influence gastric pH, fecal score, or growth performance, but reduced bone ash and serum albumin in weanling pigs

Lagos, L. Vanessa, Su A Lee, Mike R. Bedford, and Hans H. Stein. 2021. Reduced concentrations of limestone and monocalcium phosphate in diets without or with microbial phytase did not influence gastric pH, fecal score, or growth performance, but reduced bone ash and serum albumin in weanling pigs. Transl. Anim. Sci. 2021.5:1-10. doi.org/10.1093/tas/txab115.

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Effect of phytase on weaning piglet performance when fed diets supplemented with pharmacological levels of Zn

Pharmacological levels of Zn (i.e., 2,000 to 3,000 mg/kg) is often included in diets for weanling pigs to prevent post-weaning diarrhea. However, pharmacological levels of Zn may reduce microbial phytase efficacy by chelating the phytate molecule, which subsequently prevents access for phytase.  However, it is possible that this effect can be reduced by adding more phytase to diets. Therefore, an experiment was conducted to test the hypothesis that inclusion of increasing levels of phytase increases pig growth performance and mineral digestibility in diets with 3,000 mg/kg of Zn.

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Effects of reducing the concentration of Ca and P and increasing microbial phytase on gastric pH, fecal score, plasma inositol, growth performance, and bone ash of weanling pigs

The limited capacity for weanling pigs to secret HCl in the stomach may be exacerbated by inclusion of ingredients with high acid binding capacity such as limestone and monocalcium phosphate. As a consequence, reducing the amount of these 2 ingredients in diets for weanling pigs may contribute to a stable low pH for proper pepsin activity and increased action of microbial phytase. Inclusion of high doses of phytase that results in increased phytate degradation and increased release of Ca, P, and inositol may also be beneficial to newly weaned pigs. Therefore, an experiment was conducted to test the hypothesis that lowering dietary Ca and P reduces gastric pH and diarrhea of weanling pigs, but microbial phytase may overcome negative effects of low Ca and P on growth performance and bone ash.

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Formulation of diets for pigs based on a ratio between digestible calcium and digestible phosphorus results in reduced excretion of calcium in urine without affecting retention of calcium and phosphorus compared with formulation based on values for total

Lagos, L. Vanessa, Su A Lee, Mike R. Bedford, and Hans H. Stein. 2021. Formulation of diets for pigs based on a ratio between digestible calcium and digestible phosphorus results in reduced excretion of calcium in urine without affecting retention of calcium and phosphorus compared with formulation based on values for total calcium. Journal of Animal Science, 2021, Vol. 99, No. 5, 1–7. doi:10.1093/jas/skab138.

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Formulating diets based on digestible calcium instead of total calcium does not affect growth performance or carcass characteristics, but microbial phytase ameliorates bone resorption caused by low calcium in diets fed to pigs from 11 to 130 kg

Lagos, L. Vanessa, Su A Lee, Mike R. Bedford, and Hans H. Stein. 2021. Formulating diets based on digestible calcium instead of total calcium does not affect growth performance or carcass characteristics, but microbial phytase ameliorates bone resorption caused by low calcium in diets fed to pigs from 11 to 130 kg. Journal of Animal Science, 2021, Vol. 99, No. 3, 1–11. doi:10.1093/jas/skab057.

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Effect of reducing the concentration of limestone and monocalcium phosphate in diets without or with microbial phytase on gastric pH, fecal score, growth performance, and bone ash of weanling pigs

Weanling pigs have reduced secretion of HCl in the stomach needed for appropriate protein digestion. Therefore, acidifiers are sometimes used in weaning diets as alternatives to antibiotic growth promoters because these products may create a favorable environment in the stomach for proper pepsin activity. However, the presence of limestone and monocalcium phosphate (MCP) in phase 1 diets, which have a high buffer capacity, may contribute to the inability for pigs to secrete enough HCl in the stomach, and lowering the inclusion level of these ingredients in starter diets may be beneficial to young pigs. Therefore, the objective of this experiment was to test the hypothesis that reducing the amount of limestone and MCP in diets for weanling pigs, by lowering the concentration of dietary Ca and P and(or) by including microbial phytase in the diet, will reduce stomach pH and fecal score and therefore improve growth performance of pigs.

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The effect of increasing phytase dose to 1000 FTU/kg on phosphorous and calcium digestibility in pigs fed diets without inorganic P

Rundle, Carly M., Barthold Hillen, Yueming Dersjant-Li, Anne-Marie Debicki-Garnier, Hans H Stein. 2020. The effect of increasing phytase dose to 1000 FTU/kg on phosphorous and calcium digestibility in pigs fed diets without inorganic P. J. Anim. Sci. 98(Suppl. 4): 100-101. doi.org/10.1093/jas/skaa278.184. (Abstr.). Link to abstract.

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Influence of a novel consensus bacterial 6-phytase variant on mineral digestibility and bone ash in young growing pigs fed diets with different concentrations of phytate

Microbial phytase is usually included in diets for pigs to increase P absorption and utilization by hydrolyzing phytate within the gastrointestinal tract of pigs. High doses of phytase (i.e., > 1,000 FTU/kg) is also hypothesized to increase release of nutrients other than P due to increased degradation of phytate. A next generation biosynthetic bacterial 6-phytase (PhyG; DuPont Animal Nutrition) may increase digestibility of nutrients in diets for pigs; however, there are no data to demonstrate the efficacy of this phytase. Therefore, an experiment was conducted to test the hypothesis that the negative impact of phytate is reduced at higher phytase doses. It was also the objective of this research to test the hypothesis that inclusion of increasing levels of phytase increases bone ash and apparent total tract digestibility (ATTD) of minerals in diets containing varying phytate concentrations.

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Effect of formulating diets based on a ratio between STTD Ca and STTD P and the inclusion of phytase on the calcium and phosphorus balance of growing pigs

Several experiments were conducted to estimate Ca digestibility in different feed ingredients in the presence or absence of microbial phytase to allow formulation of diets for pigs to be based on standardized total tract digestible (STTD) Ca instead of total Ca. Thus, 4 experiments aimed at determining Ca requirements expressed as a ratio between STTD Ca and STTD P in pigs from 11 to 130 kg. A follow-up study was later conducted to validate those data and to evaluate the effect of using ratios that maximize growth performance on bone development because maximum bone ash requires more Ca than maximum growth performance. However, data indicate that STTD Ca to STTD P ratios to maximize Ca retention are greater than to maximize bone ash synthesis. The use of STTD Ca to STTD P ratios in diet formulation may result in a reduction in excess dietary Ca, which is beneficial because excess dietary Ca is detrimental to P digestibility and growth performance of pigs. Therefore, the objective of this experiment was to test the hypothesis that formulating diets for growing pigs based on a ratio between STTD Ca and STTD P instead of total Ca and STTD P does not decrease Ca retention, but increases P utilization.

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