Amino acids

A new source of high-protein distillers dried grains with solubles (DDGS) has greater digestibility of amino acids and energy, but less digestibility of phosphorus, than de-oiled DDGS when fed to growing pigs

Cristobal, Minoy, Jessica P. Acosta, Su A Lee, and Hans H. Stein. 2020. A new source of high-protein distillers dried grains with solubles (DDGS) has greater digestibility of amino acids and energy, but less digestibility of phosphorus, than de-oiled DDGS when fed to growing pigs. Journal of Animal Science, 2020, Vol. 98, No. 7, 1–9. doi:10.1093/jas/skaa200.

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Effects of isoquinoline alkaloids on apparent ileal digestibility of amino acids, acid hydrolyzed ether extract, and starch by young growing pigs fed corn-soybean meal diets

Rundle, Carly M., Valeria Artuso-Ponte, and Hans H. Stein. 2020. Effects of isoquinoline alkaloids on apparent ileal digestibility of amino acids, acid hydrolyzed ether extract, and starch by young growing pigs fed corn-soybean meal diets. Transl. Anim. Sci. 2020.4:1-7. doi: 10.1093/tas/txaa054. Link to full text. 

Most meat products have digestible indispensable amino acid scores that are greater than 100, but processing may increase or reduce protein quality

Bailey, Hannah M., John K. Mathai, Eric P. Berg, and Hans H. Stein. 2020. Most meat products have digestible indispensable amino acid scores that are greater than 100, but processing may increase or reduce protein quality. British Journal of Nutrition (2020), 124, 14–22. doi:10.1017/S0007114520000641. Link to full text.

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Impact of increasing the levels of insoluble fiber and on the method of diet formulation measures of energy and nutrient digestibility in growing pigs

Acosta, Jesus A., Hans H. Stein, and John F. Patience. 2020. Impact of increasing the levels of insoluble fiber and on the method of diet formulation measures of energy and nutrient digestibility in growing pigs. Journal of Animal Science, 2020, 1–9. doi:10.1093/jas/skaa130. Link to full text.

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Torula yeast has greater digestibility of amino acids and phosphorus, but not energy, compared with a commercial source of fish meal fed to weanling pigs

Lagos, Vanessa L., Hans H. Stein. 2020. Torula yeast has greater digestibility of amino acids and phosphorus, but not energy, compared with a commercial source of fish meal fed to weanling pigs. Journal of Animal Science, 2020, 1-9. doi:10.1093/jas/skz375. Link to full text.

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Effects of super dosing 4 different sources of phytase on amino acid digestibility

Dietary phytate may bind to proteins from feed ingredients by making indigestible nutrient-complexes. Therefore, it is possible that adding exogenous phytase to the diets increases digestibility of amino acids (AA). However, results of experiments in which microbial phytase has been added to diets fed to pigs have not consistently demonstrated increased ileal digestibility of AA. It is, however, possible that is because the dose of phytase was too low to obtain a positive effect on AA digestibility and that if greater doses were used, a positive response would be obtained. Therefore, the objective of this experiment was to test the hypothesis that super dosing four different sources of commercially available exogenous phytase increases the apparent ileal digestibility (AID) of CP and AA in a corn-soybean meal (SBM) based diet fed to growing pigs.

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Standardized ileal digestibility of amino acids in canola meal fed to gestating and lactating sows

Velayudhan, Deepak Ettungapladi , Manik M. Hossain, Hans H. Stein, and C. Martin Nyachoti. 2019. Standardized ileal digestibility of amino acids in canola meal fed to gestating and lactating sows. Journal of Animal Science, 2019, 4219–4226. Link to full text.

Swine Nutrition

Stein H. H. 2019. Swine Nutrition. Feedstuffs reference issue & nutrition guide. September 2019, p 35 - 40. Link to full text.

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Can the digestible indispensable amino acid score methodology decrease protein malnutrition

Bailey, H. M., H. H. Stein. 2019. Can the digestible indispensable amino acid score methodology decrease protein malnutrition. Animal Frontiers, Volume 9, Issue 4, October 2019, Pages 18–23. Link to full text.

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Effects of heat treatment on digestibility of amino acids and concentration of metabolizable energy in soybean meal fed to pigs

Lee, S. A., M. S. F. Oliveira, W. B. Kwon, and H. H. Stein. 2019. Effects of heat treatment on digestibility of amino acids and concentration of metabolizable energy in soybean meal fed to pigs. Book of Abstracts. In: 1st International Feed Technology Congress, Cologne, Germany. p. 33. (Abstr.). Link to Abstract.

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Direct vs. difference method to determine amino acid digestibility in ingredients fed to pigs

Maryane S. Faria de Oliveira, John K. Htoo, Hans H. Stein. 2019. Direct vs. difference method to determine amino acid digestibility in ingredients fed to pigs. Journal of Animal Science, Volume 97, Issue Supplement_2, July 2019, Pages 67–68. (Abstr.). Link to abstract.

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Digestibility of amino acids, energy, acid hydrolyzed ether extract, and neutral detergent fiber, and concentration of digestible and metabolizable energy in low-oil distillers dried grains with solubles fed to growing pigs

Espinosa Charmaine D., Su A. Lee, and Hans H. Stein. 2019. Digestibility of amino acids, energy, acid hydrolyzed ether extract, and neutral detergent fiber, and concentration of digestible and metabolizable energy in low-oil distillers dried grains with solubles fed to growing pigs. Transl. Anim. Sci. 2019.3:662–675. Link to full text

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Effects of Fermex 200 (fermented soybean meal) on growth performance and amino acid digestibility by weanling pigs

Soybean meal (SBM) is one of the most important protein sources in swine diets. However, SBM has high concentrations of non-digestible oligosaccharides, mainly stachyose, raffinose, and verbascose, which may increase diarrhea incidence and reduce nursery growth performance. Fermex 200 (Purina Animal Nutrition, Shoreview, MN, USA) is a new source of fermented SBM that may serve as an alternative to other protein sources in diets fed to pigs. However, there are at this point no data for effects of Fermex 200 on growth performance and amino acid (AA) digestibility when fed to weanling pigs.

Therefore, 2 experiments were conducted to test the hypothesis that the apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of AA in Fermex 200 are greater than in conventional SBM. The second hypothesis was that Fermex 200 supports growth of weanling pigs as well as other protein sources.

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Amino acid and energy digestibility of an enhanced torula yeast and fish meal fed to weanling pigs

Yeast may be used instead of fish meal or other animal protein sources in diets for weanling pigs and there are several yeast proteins available. A newly developed enhanced torula yeast is derived from forestry by-products, and therefore, has a lower carbon-footprint compared with other yeast products. This enhanced torula yeast has an improved amino acid (AA) profile compared with traditional fermentation products, but at this time no data for the nutritional value of enhanced torula yeast fed to weanling pigs have been published. Therefore, 2 experiments were conducted to test the hypothesis that the AA and energy values of enhanced torula yeast is not different from that of fish meal. In Exp. 1, the apparent ileal digestibility (AID) and the standardized ileal digestibility (SID) of AA were determined, whereas Exp. 2 was designed to determine the concentration of digestible energy (DE) and metabolizable energy (ME) in both enhanced torula yeast and fish meal.

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Effects of microbial phytase on mucin synthesis, gastric protein hydrolysis, and degradation of phytate along the gastrointestinal tract of growing pigs

Mesina Von G. R., L. Vanessa Lagos, Rommel C. Sulabo, Carrie L. Walk, and Hans H. Stein. 2019. Effects of microbial phytase on mucin synthesis, gastric protein hydrolysis, and degradation of phytate along the gastrointestinal tract of growing pigs. J. Anim. Sci. 2019.97:756–767. Link to full text.

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Effects of Bacillus amyloliquefaciens and Bacillus subtilis on ileal digestibility of AA and total tract digestibility of CP and gross energy in diets fed to growing pigs

Blavi Laia, Jens N. Jørgensen, and Hans H. Stein. 2019. Effects of Bacillus amyloliquefaciens and Bacillus subtilis on ileal digestibility of AA and total tract digestibility of CP and gross energy in diets fed to growing pigs. J. Anim. Sci. 2019.97:727–734. Link to full text.

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Effects of increasing concentrations of an Escherichia coli phytase on the apparent ileal digestibility of amino acids and the apparent total tract digestibility of energy and nutrients in corn-soybean meal diets fed to growing pigs

Yue She, J. Chris Sparks, and Hans H. Stein. 2018. Effects of increasing concentrations of an Escherichia coli phytase on the apparent ileal digestibility of amino acids and the apparent total tract digestibility of energy and nutrients in corn-soybean meal diets fed to growing pigs. J. Anim. Sci. 96:2804–2816.

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Apparent and standardized ileal digestibility of AA and starch in hybrid rye, barley, wheat, and corn fed to growing pigs

McGhee Molly L. and Hans. H. Stein. 2018. Apparent and standardized ileal digestibility of AA and starch in hybrid rye, barley, wheat, and corn fed to growing pigs. J. Anim. Sci. 96:3319–3329.  Link to full text.

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Crystalline amino acids in diets used to determine the standardized ileal digestibility of amino acids in feed ingredients fed to pigs do not influence calculated values for amino acid digestibility

Protein and amino acids quality in feed ingredient is most correctly evaluated by determining the standardized ileal digestibility (SID) of crude protein (CP) and amino acids. Experiments to determine the SID of CP and amino acids in feed ingredients often utilize semi-synthetic diets that contain the test ingredient as the sole source of CP and amino acids. Therefore, the CP and amino acid supply from experimental diets do not always meet the requirement for standardized ileal digestible amino acids by pigs and, therefore, pig growth is often limited in these experiments.

Crystalline amino acids are believed to be rapidly absorbed and 100% digestible. Addition of crystalline amino acids to diets during the adaptation or collection phase is, therefore, believed not to influence values for SID of amino acids in the test feed ingredient, but this has not been experimentally verified. Therefore, an experiment was conducted to test the hypothesis that addition of crystalline amino acids to diets during the adaptation or collection periods of digestibility studies will not influence calculated values for SID of CP and amino acids in corn and soybean meal. 

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Amino acid digestibility in six sources of meat and bone meal, blood meal, and soybean meal fed to growing pigs

Navarro D.M.D.L., J.K. Mathai, N.W. Jaworski, H.H. Stein. 2018. Amino acid digestibility in six sources of meat and bone meal, blood meal, and soybean meal fed to growing pigs. Can. J. Anim. Sci. 98: 860–867. Link to full text

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