Stein

The amino acid digestibility and digestible indispensable amino acid score for rapeseed protein isolate increases after moderate heating resulting in a protein quality similar to whey protein isolate

Rapeseed is the second most produced oilseed in the world after soybean, and after the oil is extracted, a protein-rich meal is the resulting byproduct containing greater concentrations of sulfur amino acids (AA; i.e., Met and Cys) and Lys compared with legumes and cereal grains. Rapeseed proteins have great potential as a high-quality plant-based protein for humans due to their well-balanced AA profile, high metabolic utilization of protein, a protein digestibility corrected amino acid score (PDCAAS) similar to soy and milk proteins, and easily separable antinutritional factors. However, to our knowledge, digestible indispensable amino acid score (DIAAS) has not been determined for rapeseed protein isolate and the level of processing required to concentrate rapeseed into a protein isolate warrants further evaluation of its protein quality. Therefore, this experiment was conducted to test the hypothesis that heat treatment of rapeseed protein isolate will increase the digestibility of AA by growing pigs and result in a DIAAS that is comparable to soy and animal protein isolates.

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Effects of increasing phytase dose on total tract digestibility of minerals and energy in pigs

In most plant feed ingredients, the majority of P is bound to phytate, which reduces digestibility of P in pigs, and therefore, the concentration of digestible P in these ingredients is relatively low. Phytate is negatively charged in the intestinal tract and can bind both endogenous and dietary nutrients, which results in precipitation of non-digestible nutrient-phytate complexes. Therefore, it is possible that the use of exogenous phytase can also increase the digestibility of other nutrients than P. As an example, addition of exogenous phytase to diets also releases Ca from phytate, and thus, increases the digestibility of Ca. However, it has not been conclusively demonstrated that phytase also increases the digestibility of energy-generating nutrients and other minerals in diets fed to pigs and inconsistent results among experiments have been reported. Therefore, the objective of this experiment was to test the hypothesis that increasing phytase dose increases the apparent total tract digestibility (ATTD) of minerals and gross energy (GE) in corn and soybean meal-based diets fed to growing pigs.

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Comparative digestibility and retention of calcium and phosphorus in normal- and high-phytate diets fed to gestating sows and growing pigs

Lee, S. A., M. R. Bedford, H. H. Stein. 2021. Comparative digestibility and retention of calcium and phosphorus in normal- and high-phytate diets fed to gestating sows and growing pigs. Animal Feed Science and Technology 280 (2021) 115084. doi.org/10.1016/j.anifeedsci.2021.115084. Link to full text.

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Effects of supplemental d-methionine in comparison to l-methionine on nitrogen retention, gut morphology, antioxidant status, and mRNA abundance of amino acid transporters in weanling pigs

Espinosa, Charmaine D., John K. Mathai, Laia Blavi, Yanhong Liu, John K. Htoo, J. Caroline Gonzalez-Vega, and Hans H. Stein. 2021. Effects of supplemental d-methionine in comparison to l-methionine on nitrogen retention, gut morphology, antioxidant status, and mRNA abundance of amino acid transporters in weanling pigs. Journal of Animal Science, 2021, Vol. 99, No. 9, 1–10. doi.org/10.1093/jas/skab248.

Hybrid rye may replace up to 75% of the corn in diets for gestating and lactating sows without negatively impacting sow and piglet performance

McGhee, Molly L., Hans. H. Stein. 2021. Hybrid rye may replace up to 75% of the corn in diets for gestating and lactating sows without negatively impacting sow and piglet performance. Journal of Animal Science, 2021, Vol. 99, No. 9, 1–9. doi.org/10.1093/jas/skab230. Link to full text.

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Ca and P digestibility values aren’t accurate for formulating sow diets

Lee, Su A and H. H. Stein. 2021. Ca and P digestibility values aren’t accurate for formulating sow diets. Pork Magazine, On-line edition, Sep. 9, 2021. Link to full text.

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Addition of hydrochloric acid to collection bags or collection containers did not change basal endogenous losses or ileal digestibility of amino acid in corn, soybean meal, or wheat middlings fed to growing pigs

Lee, Su A, Laia Blavi, Diego M. D. L. Navarro, and Hans H. Stein. 2021. Addition of hydrochloric acid to collection bags or collection containers did not change basal endogenous losses or ileal digestibility of amino acid in corn, soybean meal, or wheat middlings fed to growing pigs. Animal Bioscience Vol. 34, No. 10:1632-1642 October 2021. doi.org/10.5713/ab.20.0838.

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Digestibility of amino acids and concentration of metabolizable energy are greater in high-oil corn than in conventional corn when fed to growing pigs

Espinosa, C. D., N. S. Fanelli, H. H. Stein. 2021. Digestibility of amino acids and concentration of metabolizable energy are greater in high-oil corn than in conventional corn when fed to growing pigs. Animal Feed Science and Technology 280: 115040. doi.org/10.1016/j.anifeedsci.2021.115040. Link to full text.

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Digestibility of energy and concentrations of digestible and metabolizable energy in three sources of corn protein fed to weanling pigs

Corn coproducts produced from the fuel ethanol or the wet milling industries may be used in diets for pigs. Different technologies are used to develop high protein corn co-products, but in addition to providing amino acids to the diets, high protein corn co-products also provide energy to the diets. The energy concentrations in corn protein may depend on the concentrations of fat, carbohydrates, and protein in sources of corn protein. Newly developed corn proteins contain 40 to 50% crude protein. There are, however, limited data on how differences in the chemical composition of different sources of corn protein influence the concentrations of digestible energy (DE) and metabolizable energy (ME) when fed to weanling pigs. Therefore, the objective of this experiment was to test the null hypothesis that there is no difference in the apparent total tract digestibility (ATTD) of gross energy (GE) and concentrations of DE and ME among 3 sources of corn protein when fed to weanling pigs.

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Sunflower expellers have greater ileal digestibility of amino acids than sunflower meal, but there are only minor variations among different sources of sunflower meal when fed to growing pigs

Ibagon, Jimena A., Su A Lee, and Hans H. Stein. 2021. Sunflower expellers have greater ileal digestibility of amino acids than sunflower meal, but there are only minor variations among different sources of sunflower meal when fed to growing pigs. Journal of Animal Science, 2021, Vol. 99, No.

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Growth performance and carcass quality are not different between pigs fed diets containing cold-fermented low-oil DDGS and pigs fed conventional DDGS, but pelleting improves gain to feed ratio regardless of source of DDGS

Rodriguez, Diego A.,  Su A Lee, and Hans H. Stein. 2021. Growth performance and carcass quality are not different between pigs fed diets containing cold-fermented low-oil DDGS and pigs fed conventional DDGS, but pelleting improves gain to feed ratio regardless of source of DDGS. Journal of Animal Science, 2021, Vol. 99, No. 8, 1–8. doi:10.1093/jas/skab129.

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Influence of a novel consensus bacterial 6-phytase variant on mineral digestibility and bone ash in young growing pigs fed diets with different concentrations of phytate-bound phosphorus

Espinosa, Charmaine D., Maryane S. F. Oliveira, Deepak E. Velayudhan, Yueming Dersjant-Li, Hans H. Stein. 2021. Influence of a novel consensus bacterial 6-phytase variant on mineral digestibility and bone ash in young growing pigs fed diets with different concentrations of phytate-bound phosphorus. Journal of Animal Science, 2021, Vol. 99, No. 8, 1–12. doi.org/10.1093/jas/skab211.

Cheese Co-Product Offers High Nutritional Value for Weanling Pigs

Mallea, A. P., and H. H. Stein. 2021. Cheese Co-Product Offers High Nutritional Value for Weanling Pigs. Pork Magazine, On-line edition, Aug. 12, 2021. Link to full text.

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Reduced concentrations of limestone and monocalcium phosphate in diets without or with microbial phytase did not influence gastric pH, fecal score, or growth performance, but reduced bone ash and serum albumin in weanling pigs

Lagos, L. Vanessa, Su A Lee, Mike R. Bedford, and Hans H. Stein. 2021. Reduced concentrations of limestone and monocalcium phosphate in diets without or with microbial phytase did not influence gastric pH, fecal score, or growth performance, but reduced bone ash and serum albumin in weanling pigs. Transl. Anim. Sci. 2021.5:1-10. doi.org/10.1093/tas/txab115.

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Growth performance of weanling pigs fed diets with different inclusion levels of a cheese co-product

Whey, which is a co-product from dairy processing plants that extract fat and protein from milk to make cheese, has been used in diets fed to weanling pigs as a source of lactose. Whey powder does not contain much protein because the majority of the milk protein ends up in the cheese during processing. However, cheese co-products, which contain 40 to 50% crude protein may be used in the feeding of pigs, but there is limited information about the nutritional value of cheese co-products fed to pigs. Therefore, the objective of this research was to test the hypothesis that a cheese co-product may replace traditional protein sources in diets for weanling pigs without affecting growth performance.

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Standardized ileal digestibility of amino acids in three sources of corn protein fed to weanling pigs

Corn coproducts produced from the fuel ethanol or the wet milling industries may be used in diets fed to pigs. Newly developed corn proteins are high protein feed ingredients with approximately 40 to 50% crude protein (CP), and corn protein may be a great source of digestible amino acids (AA) in diets fed to weanling pigs. There are, however, limited data on the digestibility of AA in different sources of corn protein fed to weanling pigs. Therefore, the objective of this experiment was to test the null hypothesis that there is no difference in the standardized ileal digestibility (SID) of AA in 3 different sources of corn protein when fed to weanling pigs.

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Soybean meal sourced from Argentina, Brazil, China, India and USA as an ingredient in practical diets for Pacific white shrimp Litopenaeus vannamei

Galkanda-Arachchige, Harsha S. C., Hans H. Stein, and D. Allen Davis. 2021. Soybean meal sourced from Argentina, Brazil, China, India and USA as an ingredient in practical diets for Pacific white shrimp Litopenaeus vannamei. Aquaculture Nutrition. 2021;27:1103–1113. doi: 10.1111/anu.13251. Link to full text.

Corn protein has greater concentrations of digestible amino acids and energy than low-oil corn distillers dried grains with solubles when fed to pigs but does not affect the growth performance of weanling pigs

Acosta, Jessica P., Charmaine D. Espinosa, Neil W. Jaworski, and Hans H. Stein. 2021. Corn protein has greater concentrations of digestible amino acids and energy than low-oil corn distillers dried grains with solubles when fed to pigs but does not affect the growth performance of weanling pigs. Journal of Animal Science, 2021, Vol. 99, No. 7, 1–12. doi:10.1093/jas/skab175.

Effect of phytase on weaning piglet performance when fed diets supplemented with pharmacological levels of Zn

Pharmacological levels of Zn (i.e., 2,000 to 3,000 mg/kg) is often included in diets for weanling pigs to prevent post-weaning diarrhea. However, pharmacological levels of Zn may reduce microbial phytase efficacy by chelating the phytate molecule, which subsequently prevents access for phytase.  However, it is possible that this effect can be reduced by adding more phytase to diets. Therefore, an experiment was conducted to test the hypothesis that inclusion of increasing levels of phytase increases pig growth performance and mineral digestibility in diets with 3,000 mg/kg of Zn.

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Effect of dietary crude protein level on growth performance, blood characteristics, and indicators of intestinal health in weanling pigs

Limbach, Joseph R., Charmaine D. Espinosa, Estefania Perez-Calvo, and Hans H. Stein. 2021. Effect of dietary crude protein level on growth performance, blood characteristics, and indicators of intestinal health in weanling pigs. Journal of Animal Science, 2021, Vol. 99, No. 6, 1–14. doi:10.1093/jas/skab166. Link to full text.

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