Stein

Effects of excess dietary leucine in corn protein diets

Malea, A. P., and H. H. Stein. 2023. Effects of excess dietary leucine in corn protein diets. National Hog Farmer, On-line edition. November 14, 2023.

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Digestibility of calcium in calcium-containing ingredients and requirements for digestible calcium by growing pigs

Lee, Su A, L. Vanessa Lagos, Laura A. Merriman, and Hans H. Stein. 2023. Digestibility of calcium in calcium-containing ingredients and requirements for digestible calcium by growing pigs. Journal of Animal Science, 101, 1–13. doi.org/10.1093/jas/skad328. Link to full text.

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Xylanase and a stimbiotic increased growth performance and total tract digestibility of nutrients in diets for weanling pigs, but feeding sows xylanase in lactation did not influence pig growth performance

Arabinoxylans are the predominant fiber component in cereal grains and cereal co-products. They have a backbone of β-(1− 4) linked xylose units with side chains of arabinose, glucuronic acid, acetyl, and phenolic acids. Xylanase hydrolyzes the β-(1− 4) glycosidic bonds between xylose units in the backbone and releases a mixture of xylose and xylo-oligosaccharides that can be either absorbed or fermented by pigs. Previous data indicate that xylanase increased the degradation of dietary fiber and increased energy digestibility in diets for growing pigs and lactating sows. Xylo-oligosaccharides improve growth performance of nursery pigs because they serve as prebiotics that modulate gut microbiota. A stimbiotic (i.e., xylanase in combination with xylo-oligosaccharides) may improve growth performance of weanling pigs to a greater extent than either additive alone by shifting the intestinal microbiome to favor fiber fermentation. However, there are no data on possible effects of xylanase in sows diets on growth performance and intestinal health of the offspring or on the impact of the stimbiotic on digestibility of nutrients when fed to nursery pigs. Therefore, an experiment was conducted to test the hypothesis that xylanase or a stimbiotic improves growth performance and the apparent total tract digestibility (ATTD) of dry matter (DM), gross energy (GE), and total dietary fiber (TDF), and the concentration of digestible energy (DE) of diets for weanling pigs, but that the effect is greater in offspring of sows fed xylanase in lactation than in offspring of sows not fed xylanase.

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Effects of source of calcium carbonate and microbial phytase on digestibility of calcium

It is important that the digestibility of Ca in Ca sources is known to formulate diets based on values for digestible Ca rather than total Ca. Only a small amount of the Ca required by pigs is provided by plant-based ingredients and supplementation of Ca from Ca phosphates and Ca carbonate, is usually required to meet the requirement by pigs. In addition, use of microbial phytase increases Ca digestibility in Ca carbonate, which is one of the major sources of Ca in pig diets.

Differences in Ca digestibility in 4 sources of Ca carbonate produced in the United States have been observed, but it is unknown if there are differences in the ATTD of Ca in calcium carbonate sources produced outside the United States. Therefore, the objective of this experiment was to test the hypothesis that there are differences in the apparent total tract digestibility (ATTD) and standardized total tract digestibility (STTD) of Ca and in the response to microbial phytase among 20 sources of Ca carbonate obtained from different parts of the world.

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Chemical composition of banana meal and rice bran from Australia or South-East Asia

Fanelli, Natalia S., Leidy J. Torres-Mendoza, Jerubella J. Abelilla, and Hans H. Stein. 2023. Chemical composition of banana meal and rice bran from Australia or South-East Asia. Anim Biosci, Vol. 36, No. 10:1568-1577. doi.org/10.5713/ab.23.0071. Link to full text.

Digestible indispensable amino acid scores (DIAAS) for egg and plant proteins

Fanelli, Natalia dos Santos, Juliana Carolina Florencio Ravagnani Martins, and Hans Henrik Stein. 2023. Digestible indispensable amino acid scores (DIAAS) for egg and plant proteins. International Symposium Dietary Protein for Human Health. Utrecht, The Netherlands Sep 2023. Pag 74. Link to Abstract.

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Pelleting of diets for pigs improves feed efficiency

Lee. S. A., and H. H. Stein. 2023. Pelleting of diets for pigs improves feed efficiency. National Hog Farmer, On-line edition. September 27, 2023.

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Non-traditional feed ingredients in diets for pigs.

Stein, H. H. 2023. Non-traditional feed ingredients in diets for pigs. Book of abstracts of the 74th annual meeting of the European Federation of Animal Science. Aug. 26 to Sep. 1, 2023. Lyon, France. Page 286. Link to abstract.

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Short communication: Commercial diets for pigs in the United States contain more calcium than formulated.

Lagos, L. V., J. C. Woodworth, S. W. Kim, and H. H. Stein. 2023. Short communication: Commercial diets for pigs in the United States contain more calcium than formulated. J. Anim. Sci.101:1-5. doi.org/10.1093/jas/skad102. Link to full text.

 

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Digestible indispensable amino acid scores (DIAAS) in egg proteins and additivity of DIAAS in egg-style combinations

Protein malnutrition is a serious problem in both underdeveloped countries and the U.S., where many children and elderly people don't get enough protein. To determine the quality of food proteins, experts use a method called digestible indispensable amino acid score (DIAAS), which measures how well the body can digest the amino acids in a food item. The FAO recommended this method in 2011. According to the DIAAS method, protein claims can only be made for foods with a score above 75. Eggs are rich in protein and essential amino acids needed for muscle development and bodily functions. However, it's important to consider how eggs are cooked, as different methods can affect the availability of amino acids. Eggs are often eaten with other foods, and it's believed that the high-quality protein in eggs can make up for the lower quality of protein in plant ingredients when eaten together, but more research is needed to confirm this. Therefore, the objectives of this experiment were to determine DIAAS for eggs cooked in different forms and in traditional egg-style combinations with breads or hash brown, and test the hypothesis that protein quality in eggs can compensate for the low protein quality of plant-based ingredients and that DIAAS in different combined meals is additive.

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Supplementation of Valine, Isoleucine, and Tryptophan may overcome the negative effects of dietary excess Leucine in corn protein when fed to weanling pigs

High protein corn protein (HPCP) is a corn co-product derived from the ethanol industry that contains between 40 and 50% crude protein, and therefore, may be used in diets for pigs as a source of amino acids (AA). However, such diets will contain more than twice as much Leu as recommended and there is a negative relationship between dietary Leu and brain synthesis of serotonin, which results in reduced feed intake of pigs fed diets containing excess Leu. There is also a reduced protein synthesis because of increased Val and Ile metabolism due to excess dietary Leu. As a consequence, pigs often have reduced growth performance if fed diets with high concentrations of HPCP. However, it may be possible to counteract the negative effects of the high Leu concentrations in corn co-products by adding crystalline sources of Trp, Val, and Ile to the diets and it may, therefore, be possible that HPCP can be used in diets without influencing growth performance or intestinal health of weanling pigs. Therefore, the hypothesis that HPCP may be used as the primary source of AA in diets for weanling pigs if diets are fortified with crystalline AA was tested. 

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Increasing phosphorus digestibility in novel corn fermented products

Espinosa, C. D., J. D. Ward, and H. H. Stein. 2023. Increasing phosphorus digestibility in novel corn fermented products. National Hog Farmer, July 27, 2023.

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Effect of heat treatment on protein quality of rapeseed protein isolate compared with non-heated rapeseed isolate, soy and whey protein isolates, and rice and pea protein concentrates

Bailey, Hannah M., Natalia S. Fanelli, and Hans H. Stein. 2023. Effect of heat treatment on protein quality of rapeseed protein isolate compared with non-heated rapeseed isolate, soy and whey protein isolates, and rice and pea protein concentrates. J. Sci. Food Agric. 2023. doi: 10.1002/jsfa.12809. Link to full text.

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Concentration of net energy in diets containing three different sources of field peas with different particle sizes fed to group-housed growing pigs

Field peas (Pisum sativum L.) are an annual season grain legume crop and are cultivated in areas that are too cold for the cultivation of soybeans. Market opportunities for field peas have increased in recent years, and the cost of cultivation is less for peas than for soybeans. The concentration of starch in field peas is less, but crude protein and amino acids are greater than in cereal grains. Therefore, in addition to providing amino acids, field peas also provide energy to swine diets, which is important because energy is the most expensive component in diets. As a consequence, it is important to determine the energy value of field peas. Agronomic practices, growing location, and differences among varieties may impact the nutritional properties of field peas, including energy digestibility. It was also observed that in-vitro energy digestibility of field peas was increased by reducing the particle size. However, there is no information on the effects of reducing particle size on concentrations of digestible energy (DE), metabolizable energy (ME), or net energy (NE) in field peas fed to group-housed pigs. Likewise, the digestibility of energy in field peas grown in the U.S. has not been compared with the digestibility of energy of field peas grown in Canada. Therefore, the objective of this research was to test the hypothesis that the particle size of field peas and the location where field peas were grown may affect the apparent total tract digestibility (ATTD) of gross energy (GE) and concentration of NE in field peas fed to growing pigs.  

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Metabolizable energy and apparent total tract digestibility of energy and nutrients differ among samples of sunflower meal and sunflower expellers fed to growing pigs

Ibagon, Jimena A., Su A Lee, and Hans H. Stein. 2023. Metabolizable energy and apparent total tract digestibility of energy and nutrients differ among samples of sunflower meal and sunflower expellers fed to growing pigs. Journal of Animal Science, 101, 1–8. doi.org/10.1093/jas/skad117. Link to full text.

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Ferulic and coumaric acid in corn and soybean meal-based diets and in feces from pigs fed these diets

Lancheros, Jeimmy Paola, Charmaine D. Espinosa, Roelant Hilgers, Mirjam A. Kabelb, and Hans H. Stein. 2023. Ferulic and coumaric acid in corn and soybean meal-based diets and in feces from pigs fed these diets. J. Sci. Food Agric. 103: 5171–5176. doi 10.1002/jsfa.12572. Link to full text.

Concentration of net energy in corn without or with microbial phytase fed to group-housed pigs

Corn is the primary grain used in pig diets and provides most energy to the diets. Because energy is the most expensive component in diet formulation, it is critical to accurately determine energy concentrations in corn. Use of exogenous phytase in pig diets has been a standard and most phytase is expected to generate extra-phosphoric effects that result in increases in minerals, amino acid, or energy digestibility. Therefore, phytase companies have provided customers with matrix values that can be used for down specs of energy and nutrients in diet formulation. It is thus important to confirm if dietary phytase releases energy and other nutrients as suggested before using them. To our knowledge, however, there is no information on how much phytase can increase net energy (NE) in corn when fed to group-housed pigs. Therefore, the objective of this experiment was to test the hypothesis that addition of microbial phytase to a corn-based diet increased the apparent total tract digestibility (ATTD) of gross energy (GE) and concentration of NE by group-housed growing pigs.

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Nutritional value of Spanish Camelina sativa co-products for pigs

Cerisuelo, Alba, Pablo Ferrer, Ernesto Angel Gomez, Tofuko Awori Woyengo, Hans Henrik Stein, Mar Martínez, Jose Luís Cano, Olga Piquer. 2023. Nutritional value of Spanish Camelina sativa co-products for pigs. Animal Feed Science and Technology 301: 115665. doi.org/10.1016/j.anifeedsci.2023.115665. Link to full text.

Limited impacts of high doses of dietary copper on the gut bacterial metal resistome explain negligible co-selection of antibiotic resistance

Forouzandeh, Asal, Simon Bo Lassen, Julius Emil Brinck, Yan-Yan Zhou, Jiaojiao Zhu, David Solà-Oriol, Alessandra Monteiro f, Xiuli Hao, Jian-Qiang Su, Hans H. Stein, J. Francisco Pérez, Kristian K. Brandt. 2023. Limited impacts of high doses of dietary copper on the gut bacterial metal resistome explain negligible co-selection of antibiotic resistance. Science of the Total Environment 889 (2023) 164183. doi.org/10.1016/j.scitotenv.2023.164183.

Effects of corn hardness and drying temperature on digestibility of energy and nutrients in diets fed to growing pigs

Espinosa, Charmaine D., Joaquin Cabañas-Ojeda, Edgar O. Oviedo-Rondón, and Hans H. Stein. 2023. Effects of corn hardness and drying temperature on digestibility of energy and nutrients in diets fed to growing pigs. Journal of Animal Science, 101: 1–9. doi.org/10.1093/jas/skad124. Link to full text.

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